Abstract
This study aimed to evaluate the in vitro multiplication of ipe-amarelo tree in different 6- benzylaminopurine (BAP) concentrations. Nodal segments of seedlings obtained from the in vitro seeds germination were used as explants source being inoculated in nutrient broth ½ WPM containing different concentrations of BAP (0, 2, 4, 8 and 16 µM). The experiment was conducted in a completely randomized design with five treatments and 10 replications, each consisting of a vial containing two nodal segments. After 30 days of cultivation assessed the establishment percentage (development of new leaf primordia), callus formation at the explant base, rooting and number of shoots per explant. The BAP presence in the nutrient medium is not beneficial to the in vitro multiplication of ipe-amarelo tree seedlings. The BAP addition decreases the explants established percentage, rooting, and also the shoots formation, in addition to considerably increase in the calluses formation.
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