Study of in vitro shoot production of off-season Jackfruit (Artocarpus heterophyllus Lam)

Abstract

In vitro shoot production efficiency of off-season jackfruit (var. BARI jackfruit -2) plant was observed. There were 2 factors in this experiment. Factor A consisted of four benzyl amino purine (BAP) concentrations and factor B consisted of two nepthalene acetic acid (NAA) concentrations. Four BAP concentrations were 0.0 (Control), 1.0, 2.0 and 4.0 mg-L of BAP and two NAA concentrations were 1.0 and 2.0 mg-1 of NAA. The highest (85.36) percentage of explants regenerated in MS medium supplemented with the highest (4.00 mg-1) BAP concentration. The medium when fortified with 2.00 mg-1 BAP took the shortest (8.25 days) time. The highest (4.90 and 5.05, respectively) number of multiple shoots at both subcultures were obtained from the medium when enriched with 4.0 mg-1 BAP. Higher NAA concentration (2.00 mg-1) showed better performance than lower one. Number of multiple shoots increased at third subculture than second subculture in respect to both NAA concentrations. The shortest (8.17 days) time to shoot initiation was taken when 2.00 mg-1 each of BAP and NAA interacted with each other in MS medium. The longest (4.8 cm) micro shoots were obtained from the medium fortified with 4.00 mg-1 BAP and 2.00 mg/l NAA, respectively.