Abstract
Currently, northern blotting, in situ hybridizations, quantitative reverse transcription polymerase chain reaction (qRT-PCR), oligonucleotide microarrays and various electrochemical/fluorescent biosensors are the main strategies to measure aberrant microRNA expression levels. The low abundance of microRNA usually requires signal amplification steps in these methods to achieve desired sensitivity, which may demand complex procedure or equipment. The measurement may also become time-consuming. To facilitate sensitive, convenient and multiplexed detection of microRNAs, Professor Niko Hildebrandt's group at University of Paris-Sud has succeeded in developing a multiplexed microRNA time-gated fluorescence resonance energy transfer (TG-FRET) assay with isothermal and amplification-free single-step.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
