Abstract
Pedigree information is essential for the genetic improvement of traits of interests in breeding programs. In this study, twelve microsatellite loci were selected to optimize three multiplex PCR protocols for parentage assignment in grass carp (Ctenopharyngodon idella). One hundred and fifty adult fish and 252 progenies produced from three pilot groups (P1, P2, and P3) were used to examine the power of the three multiplex microsatellite PCR sets for parentage analysis. The average number of alleles (Na) per locus, observed heterozygosity (Ho), expected heterozygosity (He), and polymorphism (PIC) were 21.83, 0.883, 0.882, and 0.869, respectively. The combined exclusion power using all loci was greater than 99.99 %. Simulation analysis revealed a high assignment success rate (100 %). Parentage analysis of real offspring demonstrated that 99.6 % of all offspring were unambiguously allocated to single pairs of parents. Our results indicate that these three multiplex PCR sets could be used in pedigree reconstruction for grass carp breeding.
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