Abstract
We have developed a multiplex immunoassay test (immunochip) based on PHOSPHAN technology for the detection of immunoglobulin G to herpes simplex virus (HSV) types 1, 2 and cytomegalovirus (CMV). The immunochip consists of HSV type specific gG1 (HSV-1) and gG2 (HSV-2) recombinant antigens, the lysate antigen for detection of total IgG to both HSV types (HSV 1/2), and CMV specific chimeric recombinant antigen containing the immunodominant sequences of pp150, gB, pp28 and pp52 proteins. The sensitivity and specificity of simultaneous IgGs detection with recombinant proteins were comparable to the commercial ELISA kits regardless of the kind of investigated serum specimens (patient sera, standard serum panels). The lysate HSV antigen was as sensitive but significantly less specific, so that it could not be recommended for use as a component of the multiplex test. These results can be used as a basis for creating commercial multiplex tests intended for high-productive screening of HSV, CMV and other TORCH-infections in a clinical laboratory.
Highlights
Разработан иммуночип для определения иммуноглобулинов G к вирусам простого герпеса 1-го и 2-го типов (ВПГ-1, ВПГ-2) и цитомегаловирусу (ЦМВ) на основе технологии ФОСФАНТМ
State Research Institute of Biological Engineering, Moscow, 125424, Russian Federation; 2 Immunoscreen, Closed Joint Stock Company, Moscow, 125424, Russian Federation; 3 Ekolab, Closed Joint Stock Company, Elektrogorsk, 142530, Russian Federation We have developed a multiplex immunoassay test based on PHOSPHAN technology for the detection of immunoglobulin G to herpes simplex virus (HSV) types 1, 2 and cytomegalovirus (CMV)
The immunochip consists of HSV type specific gG1 (HSV-1) and gG2 (HSV-2) recombinant antigens, the lysate antigen for detection of total IgG to both HSV types (HSV 1/2), and CMV specific chimeric recombinant antigen containing the immunodominant sequences of pp150, gB, pp28 and pp52 proteins
Summary
Разработан иммуночип для определения иммуноглобулинов G к вирусам простого герпеса 1-го и 2-го типов (ВПГ-1, ВПГ-2) и цитомегаловирусу (ЦМВ) на основе технологии ФОСФАНТМ. Мультиплексный иммуноанализ для выявления иммуноглобулинов G к вирусам простого герпеса 1-го и 2-го типов и цитомегаловирусу на основе технологии ФОСФАН. State Research Institute of Biological Engineering, Moscow, 125424, Russian Federation; 2 Immunoscreen, Closed Joint Stock Company, Moscow, 125424, Russian Federation; 3 Ekolab, Closed Joint Stock Company, Elektrogorsk, 142530, Russian Federation We have developed a multiplex immunoassay test (immunochip) based on PHOSPHAN technology for the detection of immunoglobulin G to herpes simplex virus (HSV) types 1, 2 and cytomegalovirus (CMV). For citation: Nikitina A.V., Pomelova V.G., Osin N.S., Mardanly S.G. Multiplex immunoassay for detection of immunoglobulin G to herpes simplex virus types 1, 2 and cytomegalovirus based on PHOSPHAN technology.
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call