Abstract
In the present investigation, a micropropagation protocol has been developed for Vanilla borneensis Rolfe – a critically endangered orchid through multiple shoot regeneration. Through in vitro multiple shoot regeneration from both nodal and shoot tip explants, maximum (100%) shoot induction was observed. The minimum time required for shoot bud induction was observed from the shoot tip (5–7 days) on medium supplemented with BAP (4.44 mM) + KIN (2.32 mM) as compared to the nodal explants. Maximum multiple shoot regeneration was observed from nodal explants on the medium supplemented with BAP (4.44 mM) + TDZ (6.82 mM). However, maximum shoot length was observed on the medium supplemented with BAP (4.44 mM) + 15% CW and the number of nodes (5.27±0.33) per shoot after 90 days. Maximum (80-100%) of root initiation was observed in almost all the concentrations of NAA. The shortest time of root initiation was found on the medium supplemented with NAA (5.37 mM). Further, acclimatization period was found to be 15 days with 70% acclimatization while 60% of survivability was observed in the field condition. This efficient micropropagation method of V. borneensis could be successfully used for mass propagation as well as conservation of the critically endangered wild orchid.
Highlights
Vanilla borneensis is a monopodial terrestrial climbing orchid native to Southeast Asia (1)
A macropropagation technique has already been reported for V. borneensis (3)
The healthy rooted plantlets were taken out from the cul- decrease in growth and multiplication of shoot which can ture tubes and washed carefully and thoroughly with dis- be compared with micropropagation of V. planifolia(23-25)
Summary
ARTICLE HISTORY Received: 03 June 2021 Accepted: 03 November 2021 Available online Version 1.0 (Early Access): 21 November 2021 Version 2.0 : 01 January 2022. Publisher’s Note: Horizon e-Publishing Group remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Indexing: Plant Science Today, published by Horizon e-Publishing Group, is covered by Scopus, Web of Science, BIOSIS Previews, Clarivate Analytics, etc. See https:// horizonepublishing.com/journals/index.php/ PST/indexing_abstracting Copyright: © The Author(s). CITE THIS ARTICLE Hasnu S, Tanti B.
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call