Abstract
In vivo nucleotide-level mapping of nucleosomes in the promoter of the methanol oxidase (MOX) gene in the yeast Hansenula polymorpha is reported. The 4 nucleosomes analyzed are organized in families; they localize in alternative positions along a unique rotational phase, and the linker regions can be occupied by alternative nucleosomes. This organization underscores a substantial freedom of choice by histone octamers when nucleating on a promoter region.
Highlights
Multiple Overlapping Positions of Nucleosomes with Single in Vivo Rotational Setting in the Hansenula polymorpha RNA Polymerase II methanol oxidase (MOX) Promoter*
In order to verify this theoretical model and its in vivo effects, we have studied the in vivo distribution of nucleosomes on a RNA polymerase II promoter: the Hansenuta potymorpha methanol oxidase (MOX)l gene promoter
We have described the organization in families of nucleosomes in the H. polymorpha MOX promoter
Summary
Vol 270, No 19, Issue of May 12, pp. 11091-11097, 1995 Printed in U.S.A. Multiple Overlapping Positions of Nucleosomes with Single in Vivo Rotational Setting in the Hansenula polymorpha RNA Polymerase II MOX Promoter*. In vivo nucleotide-level mapping of nucleosomes in the promoter of the methanol oxidase (MOX) gene in the yeast Hansenula polymorpha is reported. MOX is the most abundant of these enzymes and is encoded by the MOX gene, the transcription of which is completely abolished by catabolite repression (glucose or ethanol) and strongly enhanced when the cells grow in methanol (Roggenkamp et at., 1984). Under these conditions, MOX makes out up to 30% of the soluble cell protein. Low levels of transcription starting close to UASI and upstream to the FB2 binding site have been observed, which do not lead to Mox protein. We observed that in vivo the - 3 to +1 nucleosomes localize on multiple alternative positions characterized by (i) unique rotational phase and (ii) extensive overlapping of t he a lternative extremities
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