Abstract
Methods: To determine the expression of alarin in murine brain weperformed immunohistochemistry using an affinity purified polyclonalantibody. The specificity of the immunostaining was tested by pre-absorptionoftheantiserawith10 μMofalarinandstainingbrainregionsoftransgenicGALPxDTAmice,lackingneuronsexpressingtheGALPgene.Results: Alarin-like-immunoreactivity (alarin-LI) was observed in avariety of areas through the whole murine brain. Nuclei showing highintensityofalarin-LIwerethemitralcelllayerof thebulb,theaccessoryolfactory bulb, the medial preoptic area, different nuclei of thehypothalamus, like the arcuate nucleus and the ventromedial hypotha-lamic nucleus, the locus coeruleus, the ventral chochlear nucleus, thefacial nucleus and the epithelial layer of the plexus choroideus.Discussion: These findings indicate that alarin could have aninfluence on various brain activities. The mapping of the peptide inthe CNS will be the prerequisite to design experiments to identifyfurther functions of alarin.Supported by a grant of the PMU (07/05/029) and FFG (822782/THERAPEP).doi:10.1016/j.regpep.2010.07.087A004Multiple functions of neuropeptide F (NPF) and FMRFamidein planariaN.D. Kreshchenko
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call