Abstract
Three-dimensional datasets from tissue biopsies may provide critical morphological information that is not readily obtained from traditional approaches to histology using thin physical sections of tissue. Multiphoton microscopy (MPM) provides optical sectioning with penetration into highly scattering materials, ready excitation of intrinsic tissue fluorescence, and access to nonlinear signals such as second harmonic generation (SHG). However, the penetration depth of MPM is typical limited to ∼200 microns in many tissues. We present MPM of entire intact, fixed and optically cleared mouse organs. Clearing of tissue is typically incomplete for large tissue samples, however, MPM has sufficient tolerance to scattering to image entire mouse organs. Using macro lenses with 5x magnification and 0.5 numerical aperture enables the generation of high resolution, large field-of-view datasets with imaging depths of several millimeters, sufficient to generate 3D image sets of mouse intestine, heart, lung, brain and other organs.
Published Version
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