Abstract
Using the methods of scanning cytophotometry, cytochemistry, and cytomorphometry, cells of hepatocarcinoma (HEp-G2), an adenocarcinoma of the large intestine (Caco-2), an embryonic kidney (HEK-293), a neuroblastoma (SH-SY5Y), a rabdomyosarcoma (RD), and larynx cancer (HEp-2) were studied 48, 72, and 96 h after the beginning of cultivation. The density of the monolayer, proliferative activity, the number of dead cells, the DNA content in nuclei and distribution of the cells in the population for this parameter, the total DNA content in nucleoli (in the perinucleolar chromatin), the number of nucleoli in nuclei, the distribution of cells by their number, the volume and area of the nuclear surface, and the total volume and area of the nucleolar surface were determined. The obtained data were used for a treelike cluster analysis of the cultures by the Pierson correlation. As a result of the analysis, the SH-SY5Y culture was separated into an individual cluster, while Caco-2, HEp-G2, HEK 293, HEp-2, and RD cultures were located in the tree of another cluster. The significance (weight) of parameters in the formation of a general pattern of cell cultures is not equal. It also turned out that the least transformed culture of neuroblastoma (SH-SY5Y) had no relationship with other cultures that show various degrees of similarity to one another. The cultures HEK 293, HEp-2, and RD were found to be close to one another in all parameters. Parameters had different significances in the formation of the general pattern of cell cultures. The parameters that characterized the population as a whole were of the greatest significance and included the following: density of monolayer, mitotic coefficient, and the number of dead cells. Additionally, the nuclear DNA content, the total area of the nucleolar surface, and the ratio of the nucleolar to nuclear DNA and of the total nucleolar to the nuclear areas are also of great importance. Other parameters were less significant.
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