Abstract
The generation time of the Amoeba proteus strain C was about 48 h. The relative DNA content was measured using the cytofluorometric technique in isolated nuclei over different intervals after synchronous divisions. The DNA content in the nuclei 48 h after division but prior to the next division exceeded by 2.2–2.9-fold the nuclear DNA content 1 h after division. From this it is inferred that (1) some part of the DNA is replicated in interphase more than once, and (2) not all nuclear DNA of the maternal cell is transmitted to the nuclei of daughter cells during mitosis. The DNA content of 48 h nuclei varied for different groups of amoebae taken from a mass culture at different time. This fact suggests that the DNA content in amoeba premitotic nuclei is not constant. Amplification of genes coding for ribosomal RNA (rRNA) during each amoeba cell cycle is considered as a possible interpretation.
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