Multioxide-superoxide dismutase enzyme-nanocomplexes and their antioxidant activity

Abstract

Growth of bionanotechnology requires functional materials, which can lower the costs and can be modified to the specific reaction. Conjugations of nanoparticles and enzymes form efficient products of multi-material enzyme-nanocomplexes, which can be controlled from synthesis to application and can provide predicted results. Multimetallic nanooxides of ZnO–CuO, ZnO–MnO, ZnO–MnO–CuO were obtained by precipitation method with sonication, followed by microwave process. Superoxide dismutase (SOD) was immobilized on the surface of the nanoparticles. Obtained nanomaterial-enzyme complexes had antioxidant properties. Particles were characterised by XRD, SEM and TEM methods and ATR spectroscopy which proved enzyme-nanooxides conjugation. Scavenging activity of the materials was on average 85% in DPPH (2,2-diphenyl-1-picrylhydrazyl) method and 20 mg/l in TROLOX (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) concentration, where pure SOD enzyme presented around 90% and 10 mg/l activity, respectively. Conjugation of the highest antioxidant power is ZnO–MnO with SOD, however all three types of materials could be used in further applications.Graphical abstract