Abstract
Among recent progress of optical bioimaging techniques, nonlinear optical imaging is one of the promising new-generation bioimaging methods. Nonlinear optical imaging is capable of rapid image acquisition, three-dimensional sectioning, label-free analysis, and simultaneous multimodal imaging. The multimodality of nonlinear optical processes enables us to obtain various signals simultaneously such as second harmonic generation (SHG), third harmonic generation (THG), third-order sum frequency generation (TSFG), and coherent anti-Stokes Ra-man scattering (CARS) [1]. Since images obtained by each process are based on different contrast mechanism, the combinational analysis of various nonlinear optical processes provides more detailed information on the sample. In the present study, we report recent our development of multimodal nonlinear spectral microscope and its applications to imaging of tissue samples. In particular, multimodal imaging with spectral analysis of the CARS molecular fingerprint provided molecular structural information of the tissue sample.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call