Abstract
Because the lack of typing tools for Cyclospora cayetanensis has hampered outbreak investigations, we sequenced its genome and developed a genotyping tool. We observed 2 to 10 geographically segregated sequence types at each of 5 selected loci. This new tool could be useful for case linkage and infection/contamination source tracking.
Highlights
Because the lack of typing tools for Cyclospora cayetanensis has hampered outbreak investigations, we sequenced its genome and developed a genotyping tool
Five microsatellite loci (CYC3, CYC13, CYC15, CYC21, and CYC22) exhibiting high PCR amplification efficiency and nucleotide sequence polymorphism in the initial evaluation were chosen for further evaluations of the nature of nucleotide sequence polymorphism by using a total of 64 C. cayetanensis specimens from China (n = 26), Nepal (n = 3), Indonesia (n = 1), Guatemala (n = 2), Peru (n = 8), Spain (n = 1), and the United States (n = 23)
In this study, we sequenced the genome of C. cayetanensis protozoa and developed a genotyping tool
Summary
We observed 2 to 10 geographically segregated sequence types at each of 5 selected loci This new tool could be useful for case linkage and infection/contamination source tracking. Five microsatellite loci (CYC3, CYC13, CYC15, CYC21, and CYC22) exhibiting high PCR amplification efficiency and nucleotide sequence polymorphism in the initial evaluation were chosen for further evaluations of the nature of nucleotide sequence polymorphism by using a total of 64 C. cayetanensis specimens from China (n = 26), Nepal (n = 3), Indonesia (n = 1), Guatemala (n = 2), Peru (n = 8), Spain (n = 1), and the United States (n = 23) (online Technical Appendix Table 2). Nucleotide sequence alignment led to the identification of 4 sequence types at locus CYC3, 10 at locus CYC13, 2 at locus CYC15, 8 at locus CYC21, and 4 at locus CYC22 (online Technical Appendix Table 2). Primer sequences of microsatellite loci used in multilocus sequence analysis of Cyclospora cayetanensis
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