Abstract
Amnion, which is usually discarded as medical waste, is considered as abundant sources for mesenchymal stem cells. In human and veterinary medicine, the multipotency of mesenchymal stem cells derived from amnion (AMSCs) together with their plasticity, self-renewal, low immunogenicity and nontumorigenicity characteristics make AMSCs a promising candidate cell for cell-based therapies and tissue engineering. However, up till now, the multipotential characteristics and therapeutic potential of AMSCs on preclinical studies remain uncertain. In this work, we successfully obtained AMSCs from Beijing duck embryos in vitro, and also attempted to detect their biological characteristics. The isolated AMSCs were phenotypically identified, the growth kinetics and karyotype were tested. Also, the cells were positive for MSCs-related markers (CD29, CD71, CD105, CD166, Vimentin and Fibronection), while the expression of CD34 and CD45 were undetectable. Additionally, AMSCs also expressed the pluripotent marker gene OCT4. Particularly, when appropriately induced, AMSCs could be induced to trans-differentiate into adipocytes, osteoblasts, chondrocytes and neurocytes in vitro. Together, these results demonstrated that the isolated AMSCs maintained their stemness and proliferation in vitro, which may be useful for future cell therapy in regenerative medicine.
Highlights
To date, stem cell research has meant a tremendous advance for cell therapy and tissue engineering (Alizadeh et al 2016; Bai et al 2016; Gao et al 2016; Guo et al 2017; Zhang et al 2016)
When appropriately induced, AMSCs could be induced to trans-differentiate into adipocytes, osteoblasts, chondrocytes and neurocytes in vitro. These results demonstrated that the isolated AMSCs maintained their stemness and proliferation in vitro, which may be useful for future cell therapy in regenerative medicine
AMSCs were successfully isolated from amnion tissues of 14-day-old Beijing duck embryos and were expanded until passage 18
Summary
Stem cell research has meant a tremendous advance for cell therapy and tissue engineering (Alizadeh et al 2016; Bai et al 2016; Gao et al 2016; Guo et al 2017; Zhang et al 2016). The amnion is filled with fluid composed of basement layer, compact layer, fibroblastic layer and spongy layer, which is a source of important mesenchymal stem cells with pluripotential characteristics (Cai et al 2010). Intensive research efforts have been reported that the AMSCs are derived from the spongy layer, which, in cell based therapies, have advantage over adult type stem cells, such as a higher in vitro expansion potential, telomerase activity, immunological tolerance (Roubelakis et al 2012). The avian eggs characterized by small body size, ease of manipulation and a low maintenance cost may serve as significant model system for stem cell research (Li et al 2011). Our present study aimed to isolate AMSCs from 14-day old Beijing duck embryos and examine their biological characteristics with regard to growth kinetics, karyotype, immunophenotype, specific mesenchymal markers and differentiation potential
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