Honey Bee Venom Will Differentiate Mesenchymal Stem Cells in to the Osteocyte

Abstract

Umbilical cord (UC) is an important source of multipotential mesenchymal stem cells (MSCs). It has observed that bee venom (BV) is effective in survival and differentiation of the cells. We hypotheses that BV can cause differentiation of MSCs to osteocytes. The cells obtained from mouse UC tissues were digested and suspended in DMEM medium. After 24 hours, to induce osteogenic differentiation, cells were cultured for 14 and 21days respectively in DMEM medium contain different concentrations of BV (1,2,3,4,5,6 µgr/ml). Following the treatment, calcium’s level in the cells was determined by Alizarin red staining. Cytotoxic effects of BV on MSCs were tested by MTT assay which are shown that BV inhibits MSCs growth. Furthermore, by Alizarin red test, we found that BV increases calcium level in MSCs on dose and time dependent manner. In conclusion we suggest that the MSCs from UC have differentiation potential to osteocyte under BV treatment and it may be useful in cell therapy.