Multigenerational effects of a complex urban contaminant mixture on the behavior of larval and adult fish in multiple fitness contexts

Abstract

Agricultural and urban storm water runoffs can introduce chemicals of emerging concern (CECs) into waterways. These chemicals can be continually released, persist, or even accumulate over time, with adverse effects on the physiology and behavior of aquatic species. Most studies aimed at evaluating the intergenerational effects of CECs have focused exclusively on single chemicals. By comparison, little is known about the effects of complex CEC mixtures on the behavior of organisms, or how these effects might manifest in subsequent generations. In this study, we exposed three generations of fathead minnows (Pimephales promelas) to environmentally relevant concentrations of a complex CEC mixture representative of urban-impacted waterways and assessed the growth and behavior of larval and adult fish in life-stage–relevant fitness contexts (foraging, boldness, courtship). We found that (i) multigenerational exposure to a complex mixture of CECs altered the behavior of both larvae and adults in different fitness contexts; (ii) concentration-dependent patterns of behavioral impairment were consistent across fitness contexts and life stages; and (iii) the effects of exposure were magnified in the F1 and F2 generations. These results highlight the need for long-term, multigenerational assessments of CECs in affected waterways to robustly inform conservation practices aimed at managing aquatic systems.