Multi-environment field testing for identification and validation of genetic resistance to Botrytis cinerea causing Botrytis grey mold in chickpea (Cicer arietinum L.)

Abstract

Botrytis grey mould (BGM), caused by Botrytis cinerea Pers. Ex. Fr., is a destructive foliar disease of chickpea (Cicer arietinum L.) worldwide. Disease management through host-plant resistance is the most effective and economic option to manage this disease. The objective of this study was to identify new sources of resistance to BGM, validate their stability across environments and determine the magnitude of G × E interaction. One hundred and nine chickpea genotypes with moderate levels of resistance (BGM severity ≤5.0 on a 1–9 scale) were selected from the preliminary evaluation of 412 genotypes including germplasm and breeding lines under controlled environmental conditions in 2004–2005 at the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India. In order to validate resistance stability, an ‘International Botrytis Grey Mould Nursery’ (IBGMN) was constituted with 25 genotypes and tested in multi-environments for BGM resistance at two locations (Gurdaspur and Pantnagar) in India for 4 years and two locations (Tarahara and Rampur) in Nepal for 3 years. Additive main effects and multiplicative interaction (AMMI) analysis showed significant genotype (G), environment (E) and G × E interaction (p < 0.0001) with largest contribution by environment (47.36%). The first two principal component axes were significant, and contributed 48.21% to the total G × E interaction. The AMMI biplot analysis allowed the selection of five genotypes ICCV 96859, ICCV 96853, ICCV 05604, ICCV 96852 and ICCV 05605 with low BGM severity (between 3.7 and 4.7 on 1–9 scale) and moderate stability. Genotype ICCV 96859 having least disease severity and moderate stability could be highlighted and exploited in chickpea resistance breeding programmes.