Multidrug Resistant Staphylococcus aureus Isolated from UTI Site and its Biofilm Inhibition Activity with Nanomaterials in Reference with Standard Antibiotics

Abstract

Listen

Translate article

Drug-resistant Staphylococcus aureus is a leading cause of nosocomial urinary tract infections and biofilm-associated illnesses worldwide. The present investigation intended to detect biofilm-forming Staphylococcus aureus, their antibiotic resistance, and plasmid profile. Thirty clinical urine samples were collected and tested for pathogen, drug-resistance pattern, plasmids profile, biofilm development, and molecular characterisation. Four strains of Staphylococcus aureus were discovered, according to Bergey's handbook. Kirby-Bauer technique was used to test antibiotic sensitivity and resistance. Four multidrug-resistant Staphylococcus aureus plasmids were examined by alkaline lysis. Among the four isolates, plasmid band was assigned, and additional investigations were employed for biofilm activity against four nanocomposite MoSe2, Mn3O4, Nb2O5 and ZnO-SnO2. Nanocomposite Nb2O5 inhibited biofilm at lower concentrations than cefotaxime. DNA was extracted and PCR was performed using the 16S rRNA gene for Staphylococcus aureus. Isolate phylogeny was analysed using Neighbor joining and uploaded to GenBank with accession number MG263510. Staphylococcus aureus multidrug resistance and biofilm formation are major medical challenges. Despite rising biofilm development rates, it is hard to compare, and hospitals have implemented tight infection control procedures. This study indicated that nanomaterials may help manage biofilmrelated illnesses