Multi-walled carbon nanotubes change morpho-functional and GABA characteristics of mouse cortical astrocytes

Abstract

BackgroundMulti-walled carbon nanotubes (MW-CNTs) have been extensively explored for their possible beneficial use in the nervous system. CNTs have shown to modulate neuronal growth and electrical properties, but its effect that varying length of MW-CNTs on primary astrocyte roles have not been clearly demonstrated yet.ResultsWe investigate here the effect of MW-CNTs on astrocytic morphology, cell–cell interaction and the distribution of intracellular GABA (gamma-amino butyric acid). Primary cultured cortical astrocytes on MW-CNT-coated glass coverslips grow rounder and make more cell–cell interactions, with many cell processes, compared to astrocytes on poly-d-lysine (PDL) coverslips. In addition, intracellular GABA spreads into the cell processes of astrocytes on MW-CNT coverslips. When this GABA spreads into cell processes from the cell body GABA can be released more easily and in larger quantities compared to astrocytes on PDL coverslips.ConclusionsOur result confirm that MW-CNTs modulate astrocytic morphology, the distribution of astrocytic GABA, cell–cell interactions and the extension of cell processes. CNTs look to be a promising material for use neuroprosthetics such as brain-machine interface technologies.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-015-0152-y) contains supplementary material, which is available to authorized users.