Abstract
A flavoenzyme, glucose-3-dehydrogenase (G3DH) from Halomonas sp. α-15 strain, possessed broad substrate specificity and site-specific reaction against various sugars. We constructed an amperometric enzyme electrode employing G3DH and also developed a multi-sugar analysis system combined with the G3DH sensor and sugar analysis chromatography. This system can discriminate the 4 components of sugar, i.e., glucose, 1,5-anhydro-D-glucitol, trehalose, and N-carbamoyl-β-D-glucopyranosylamine mixture and it can determine each concentration from 2 mM to 10 mM.
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