Multi-mode Microscopic Hyperspectral Imager for the Sensing of Biological Samples

Zhanpeng Xu,Yiming Jiang,Sailing He

doi:10.3390/app10144876

Zhanpeng Xu, Yiming Jiang + Show 1 more

Open Access

https://doi.org/10.3390/app10144876

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Journal: Applied Sciences	Publication Date: Jul 16, 2020
Citations: 20	License type: CC BY 4.0

Affiliation: Zhejiang University, Ningbo University

Abstract

In this work, we develop a multi-mode microscopic hyperspectral imager (MMHI) for the detection of biological samples in transmission imaging, reflection imaging and fluorescence mode. A hyperspectral image cube can be obtained with 5 μm spatial resolution and 3 nm spectral resolution through push-broom line scanning. To avoid possible shadows produced by the high magnification objective with a short working distance, two illumination patterns are designed to ensure the co-axiality of the illumination and detection. Three experiments for the detection of zebrafish and fingerprints and the classification of disaster-causing microalgae verify the good capability and functionality of the system. Based on the detected spectra, we can observe the impacts of β-carotene and melanin in zebrafish, hemoglobin in the fingertip, and chlorophyll in microalgae, respectively. Multi-modes can be switched freely according to the application requirement and characteristics of different samples, like transmission mode for the transparent/translucent sample, reflection mode for the opaque sample and fluorescence mode for the fluorescent sample. The MMHI system also has strong potential for the non-invasive and high-speed sensing of bio or clinical samples.

Highlights

The appearance of microscopy opens the door for humans to explore the micro world, and spectral analysis provides the intrinsic absorption or fingerprint characteristics of specific substances [1]
The spectral information can be used in point-to-point spectral analysis for interested regions in the 2D hyperspectral spatial image
We present a multi-mode microscopic hyperspectral imager (MMHI) with 5 μm spatial resolution and 3 nm spectral resolution that works in the transmission mode, reflection mode and fluorescence mode

Summary

Introduction

The combination of hyperspectral technology and optical microscopic imaging in bio-sensing has been widely used in tumor cell detection [2], food detection [3], zooplankton classification [4]. In these applications, the separation of a microscopic imaging module and a hyperspectral detection module may cause the detection errors and increase the cost of the system. Hyperspectral imaging (HSI) technology, with the advantage of acquiring two-dimensional image and corresponding spectrum of each pixel, has strong potential for, e.g., on-site monitoring, clinic diagnose and biomedicine [6,7,8]. A hyperspectral cube containing both spatial and spectral information can be obtained. The spectral information can be used in point-to-point spectral analysis for interested regions in the 2D hyperspectral spatial image

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