MP89-10 CAVEOLIN REGULATION OF PENILE HEMODYNAMICS: POSSIBLE ROLE IN ERECTILE DYSFUNCTION

Abstract

You have accessJournal of UrologySexual Function/Dysfunction: Basic Research & Pathophysiology II1 Apr 2016MP89-10 CAVEOLIN REGULATION OF PENILE HEMODYNAMICS: POSSIBLE ROLE IN ERECTILE DYSFUNCTION Jay Parikh, Dimosthenis Giamouridis, Tung-Chin (Mike) Hsieh, Jan Schilling, Valmik Bhargava, Hemal Patel, and M. Raj Rajasekaran Jay ParikhJay Parikh More articles by this author , Dimosthenis GiamouridisDimosthenis Giamouridis More articles by this author , Tung-Chin (Mike) HsiehTung-Chin (Mike) Hsieh More articles by this author , Jan SchillingJan Schilling More articles by this author , Valmik BhargavaValmik Bhargava More articles by this author , Hemal PatelHemal Patel More articles by this author , and M. Raj RajasekaranM. Raj Rajasekaran More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.2471AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Pathophysiology of age-related penile hemodynamic impairment is unclear. A defective vascular endothelium and consequent impairment in formation/action of nitric oxide (NO) are implicated as possible causes. Major NO synthesizing machinery (eNOS) is localized to caveolae (plasma membrane lipid rafts) and age-related alterations to its main structural components (caveolin 1, 3) are recognized to play a role in geriatric cardiovascular dysfunctions. Our objectives are to evaluate the role of caveolins in: (i) penile blood flow and (ii) microvascular perfusion using Cav- 1 or 3 KO mouse models. METHODS We used wild type C57BL6, Cav-1 and Cav-3 KO male mice (age 18-20 months; n=8 each). Animals were anesthetized (5% isoflurane) and penile blood flow (peak systolic velocity-PSV & end diastolic velocity EDV; mm/sec) was determined using a high-frequency/ high-resolution digital imaging color Doppler system with a 32-56 MHz linear probe (FUJIFILM VisualSonics Inc, Toronto, Canada). Penile tissue microcirculatory blood perfusion (perfusion units- PU) was measured using a novel PeriCam PSI system. Blood flow and perfusion measurements were made before and after an intracavernosal injection of vasoactive agents- PGE1 (50 ng) as well as DEA-NO (3 µg). RESULTS Representative tracings showing penile blood flow (panel A) and perfusion (panels B-C) measurements are shown in the figure. A significant impairment in hemodynamic response to PGE1 in PSV (33±4 in Cav-1KO mice compared to 62 ± 5 in WT; p<0.05) as well as perfusion (146±12 in Cav-1KO mice compared to 256 ± 12 in WT; p<0.05) was observed in Cav-1 deleted animals. Cav-3 deletion had no significant impact on penile hemodynamic changes. CONCLUSIONS Altered penile blood flow and microcirculation after Cav-1 depletion suggests a regulatory role for this protein in penile hemodynamics. Caveolin re-expression in the aging penis may be a novel approach to improve penile hemodynamics for the treatment of ED in the geriatric population. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e1140 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Jay Parikh More articles by this author Dimosthenis Giamouridis More articles by this author Tung-Chin (Mike) Hsieh More articles by this author Jan Schilling More articles by this author Valmik Bhargava More articles by this author Hemal Patel More articles by this author M. Raj Rajasekaran More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...