MP88-19 URINARY MICROBIOME PATTERNS APPEARS TO BE CORRELATIVE TO INTRAVESICAL RECURRENCE OF NON-MUSCLE INVASIVE BLADDER CANCER

Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology V1 Apr 2016MP88-19 URINARY MICROBIOME PATTERNS APPEARS TO BE CORRELATIVE TO INTRAVESICAL RECURRENCE OF NON-MUSCLE INVASIVE BLADDER CANCER Yige Bao, Hassan Razvi, Gregory Gloor, Gregor Reid, John Denstedt, and Jeremy Burton Yige BaoYige Bao More articles by this author , Hassan RazviHassan Razvi More articles by this author , Gregory GloorGregory Gloor More articles by this author , Gregor ReidGregor Reid More articles by this author , John DenstedtJohn Denstedt More articles by this author , and Jeremy BurtonJeremy Burton More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.2437AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Several studies have recently reported association between various urinary conditions. The objective of this study was to describe the urinary microbiome among bladder cancer patients, observe longitudinal urinary microbiome changes during surgery and chemotherapy, and identify microbiome patterns that are associated with frequent bladder cancer recurrences. METHODS After optimising urine sample collection, storage and processing methods for microbiome analysis, we carried out a prospective, multicenter cohort study, recruiting patients with newly diagnosed Ta, T1 or CIS transitional cell carcinoma of the bladder who underwent transurethral resection with or without intravesical instillation. Urine samples were collected at multiple time points before and after surgery as the patients were followed up for recurrence. Routine urine culture, enhanced culture, qPCR quantification and V4 16S rRNA gene sequencing were performed on urine samples for microbiome analysis. Urine samples were also analysed from gender and age-matched healthy volunteer controls. RESULTS To eliminate “background” 16S rRNA amplification we found it necessary to collect at least 20 ml of urine and store the pellet in -80 °C. We also needed to treat samples with PMA to bind any contaminating environmental DNA present. Ninety-nine patients were recruited from two medical centers and followed up for up to three years. When compared to healthy individuals, bladder cancer patients had urinary microbiome with increased alpha (within sample) diversity with a decreased proportion of “typically” commensal species. Healthy individuals generally maintained a stable urinary microbiome during the longitudinal follow up, while cancer patients’ urinary microbiome were disrupted during periods following surgery and subsequent chemotherapy, though often a restored community profile was observed in the follow up. A common urinary microbiome pattern was identified among patients with frequent recurrences compared to patients with no recurrence. CONCLUSIONS The urinary microbiome maybe indicative of likely recurrence among non-muscle invasive bladder cancer patients © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e1136 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Yige Bao More articles by this author Hassan Razvi More articles by this author Gregory Gloor More articles by this author Gregor Reid More articles by this author John Denstedt More articles by this author Jeremy Burton More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...