MP8-01 THE ESTABLISHMENT OF AN UNDERACTIVE BLADDER MODEL FOLLOWING A HYSTERECTOMY IN MONKEY

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INTRODUCTION AND OBJECTIVES: Underactive bladder (UAB) syndrome describes a dysfunctional condition of the bladder where patients are unable to produce an effective voiding contraction. To date a pathophysiological mechanisms are not fully understood. To address this issue, we established on underactive bladder model in monkeys after having a hysterectomy. METHODS: Female cynomolgus monkeys were anesthetized with ketamine chloride and pentobarbital sodium. The uterine corpus was then surgically removed while preserving the uterine cervix. Days after surgery, conscious animals were placed on chairs to monitor natural urination after oral administration of 150 mL water (uroflowmetry, UFM). In addition to this, in order to confirm the detrusor underactivity, pressure flow study (PFS) was conducted in the conscious animals. An in vitro muscle strip study was also performed. RESULTS: Monkeys showed typical detrusor underactivity in PFS. Maximum flow rate (Qmax), average flow rate (Qave) and voided volume per micturition were significantly decreased at 2, 4, 6, 8, 21, 60 days after surgery compared with before surgery. On the contrary, voiding time per micturition was significantly increased. Sham-operated group did not show any changes. In muscle strip studies, there was significant decrease in maximum response to potassium chloride (KCl) in the 2 months group but not in the 2 weeks group compared with the sham group. There was a significant decrease in the response to carbachol (CCh) and electric field stimulation (EFS) in both the 2 weeks and 2 months group. CONCLUSIONS: This is the first report on UAB model following a hysterectomy in monkeys. This model had an acutely neurogenic but chronically neurogenic and myogenic characteristics. It seems to be useful in the pathophysiological elucidation of UAB and have potential for assessment of pharmacotherapy of UAB. Source of Funding: none