MP72-06 QUANTITATIVE ANALYSIS OF MAST CELL INFILTRATION IN INTERSTITIAL CYSTITIS-IS IT STILL SPECIFIC TO INTERSTITIAL CYSTITIS?

Abstract

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Interstitial Cystitis1 Apr 2016MP72-06 QUANTITATIVE ANALYSIS OF MAST CELL INFILTRATION IN INTERSTITIAL CYSTITIS-IS IT STILL SPECIFIC TO INTERSTITIAL CYSTITIS? Yoshiyuki Akiyama, Daichi Maeda, Teppei Morikawa, Akiteru Goto, Yasuhiko Igawa, Masashi Fukayama, and Yukio Homma Yoshiyuki AkiyamaYoshiyuki Akiyama More articles by this author , Daichi MaedaDaichi Maeda More articles by this author , Teppei MorikawaTeppei Morikawa More articles by this author , Akiteru GotoAkiteru Goto More articles by this author , Yasuhiko IgawaYasuhiko Igawa More articles by this author , Masashi FukayamaMasashi Fukayama More articles by this author , and Yukio HommaYukio Homma More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.1619AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Mast cell infiltration has been documented as a key pathological feature of interstitial cystitis (IC). However, previous evidence is based on eye-measured/semi-quantitative mast cell counts, which can often be inaccurate and arbitrary. In this study, we used image analysis software to precisely count the number of infiltrating mast cells in IC biopsy specimens. In addition, by comparing IC specimens and non-IC specimens, we attempted to determine whether mast cell infiltration is an IC-specific histological feature. METHODS We quantified mast cells by immunohistochemistry in urinary bladder biopsy specimens taken from 27 patients with Hunner type IC (HIC) (n = 54, one each from the lesion and a non-lesion area), 39 patients with non-Hunner type IC (NHIC) (n = 39), and 15 non-IC patients with chronic cystitis (n = 23, including 8 from non-cancerous area of bladder cancer) using image analysis software. Mast cells were identified by immunostaining with anti-mast cell tryptase antibody. For specimens including detrusors, mast cells in the detrusors were evaluated separately in the same manner. RESULTS No significant differences in the number of mast cells were observed between HIC and non-IC cystitis specimens. In NHIC specimens, mast cell count was significantly lower than HIC and non-IC cystitis specimens (Fig. 1A). Detrusors were included in 6, 11, 21 and 15 specimens from the lesion of HIC, a non-lesion area of HIC, NHIC and non-IC cystitis, respectively. There were no significant differences in the number of mast cells in detrusors between IC and non-IC cystitis biopsy specimens (Fig. 1B). CONCLUSIONS The results indicate that there is no increase in the number of mast cell tryptase-positive cells in either entire specimen or detrusor areas of IC biopsy specimens compared to non-IC biopsy specimens, suggesting that mast cell infiltration is not a specific histological feature of IC in biopsy specimens. The diagnostic significance of mast cell counts in IC biopsy specimens is questionable. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e954 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Yoshiyuki Akiyama More articles by this author Daichi Maeda More articles by this author Teppei Morikawa More articles by this author Akiteru Goto More articles by this author Yasuhiko Igawa More articles by this author Masashi Fukayama More articles by this author Yukio Homma More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...