MP68-10 OOCYTE ACTIVATING ABILITY OF THE SPERMATOZOA APPEARS TO DECREASE WITH AGING AMONG SUBFERTILE PATIENTS

Abstract

You have accessJournal of UrologyInfertility: Evaluation1 Apr 2014MP68-10 OOCYTE ACTIVATING ABILITY OF THE SPERMATOZOA APPEARS TO DECREASE WITH AGING AMONG SUBFERTILE PATIENTS Takeshi Shin, Mai Fukushima, Toshiyuki Iwahata, Keisuke Suzuki, Yuko Sadaoka, Shigeyuki Ota, Masashi Iijima, Ryo Sato, Kojiro Nishio, Makoto Kawaguchi, Yoshitomo Kobori, Yoshio Ashizawa, Hiroshi Yagi, Shigehiro Soh, Gaku Arai, and Hiroshi Okada Takeshi ShinTakeshi Shin More articles by this author , Mai FukushimaMai Fukushima More articles by this author , Toshiyuki IwahataToshiyuki Iwahata More articles by this author , Keisuke SuzukiKeisuke Suzuki More articles by this author , Yuko SadaokaYuko Sadaoka More articles by this author , Shigeyuki OtaShigeyuki Ota More articles by this author , Masashi IijimaMasashi Iijima More articles by this author , Ryo SatoRyo Sato More articles by this author , Kojiro NishioKojiro Nishio More articles by this author , Makoto KawaguchiMakoto Kawaguchi More articles by this author , Yoshitomo KoboriYoshitomo Kobori More articles by this author , Yoshio AshizawaYoshio Ashizawa More articles by this author , Hiroshi YagiHiroshi Yagi More articles by this author , Shigehiro SohShigehiro Soh More articles by this author , Gaku AraiGaku Arai More articles by this author , and Hiroshi OkadaHiroshi Okada More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.2189AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES It has been established that increasing maternal age leads to a higher risk of infertility and miscarriage. On the other hand, the recent rise in birth rates to older fathers is of concern because of evidence that advanced paternal age might be associated with an increased risk of pregnancy and various disorders of the newborn. However there is no clearly accepted consensus on the implication of advancing male age. To examine the question of whether advanced paternal age impact fertility, we focused on oocyte activating ability of the spermatozoa and investigated age related change of this ability using mouse oocyte activation test (MOAT). METHODS MOAT, which was first proposed in 1995 by Rybouchkin et al., was used in the present study. After obtaining institutional review board approval, the infertile sperms were obtained from male infertile patients between October 2012 and September 2013. As the control, fertile sperms were obtained from donors with proven fertility. Human sperms were injected into mouse oocytes by a piezo-driven unit and these oocytes were incubated at 37°C under 5% CO2 in air. Approximately 10 hours after injection, the oocytes were observed under an microscope and were rotated to determine whether or not a second polar body was present. An oocyte with the second polar body was recorded ′activated′. We evaluated the activated rates of each generation of male infertile patients and the controls. RESULTS Semen samples from 77 male infertile patients and 15 control people were available for analysis. In the control group, the activated rates of the oocytes were 82.0% at <30 years, 80.0% at 30-34 years, 77.0% at 35-39 years, 70.0% at 40-44 years and 70.0% at 45-50 years, whereas in male infertile group 69.0% at <30 years, 68.8% at 30-34 years, 62.2% at 35-39 years, 54.8% at 40-44 years and 47.3% at 45-50 years. In both group, there was an age-associated decrease in the rate of activated oocytes. The decrease was more remarkable in male infertile group compared to the control group. CONCLUSIONS Oocyte activation ability of spermatozoa appears to decrease with aging, in particular, among subfertile patients. This suggests that we need immediate assessment and appropriate intervention when handling male infertile patients. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e802 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Takeshi Shin More articles by this author Mai Fukushima More articles by this author Toshiyuki Iwahata More articles by this author Keisuke Suzuki More articles by this author Yuko Sadaoka More articles by this author Shigeyuki Ota More articles by this author Masashi Iijima More articles by this author Ryo Sato More articles by this author Kojiro Nishio More articles by this author Makoto Kawaguchi More articles by this author Yoshitomo Kobori More articles by this author Yoshio Ashizawa More articles by this author Hiroshi Yagi More articles by this author Shigehiro Soh More articles by this author Gaku Arai More articles by this author Hiroshi Okada More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...