MP68-11 SUCCESS RATES OF NATURAL CONCEPTION AND INTRA-UTERINE INSEMINATION IN MEN WITH SEVERELY ABNORMAL STRICT MORPHOLOGY (

Abstract

INTRODUCTION AND OBJECTIVES: It has been established that increasing maternal age leads to a higher risk of infertility and miscarriage. On the other hand, the recent rise in birth rates to older fathers is of concern because of evidence that advanced paternal age might be associated with an increased risk of pregnancy and various disorders of the newborn. However there is no clearly accepted consensus on the implication of advancing male age. To examine the question of whether advanced paternal age impact fertility, we focused on oocyte activating ability of the spermatozoa and investigated age related change of this ability using mouse oocyte activation test (MOAT). METHODS: MOAT, which was first proposed in 1995 by Rybouchkin et al., was used in the present study. After obtaining institutional review board approval, the infertile sperms were obtained from male infertile patients between October 2012 and September 2013. As the control, fertile sperms were obtained from donors with proven fertility. Human sperms were injected into mouse oocytes by a piezo-driven unit and these oocytes were incubated at 37 C under 5% CO2 in air. Approximately 10 hours after injection, the oocytes were observed under an microscope and were rotated to determine whether or not a second polar body was present. An oocyte with the second polar body was recorded 0activated0. We evaluated the activated rates of each generation of male infertile patients and the controls. RESULTS: Semen samples from 77 male infertile patients and 15 control people were available for analysis. In the control group, the activated rates of the oocytes were 82.0% at <30 years, 80.0% at 30-34 years, 77.0% at 35-39 years, 70.0% at 40-44 years and 70.0% at 45-50 years, whereas in male infertile group 69.0% at <30 years, 68.8% at 30-34 years, 62.2% at 35-39 years, 54.8% at 40-44 years and 47.3% at 45-50 years. In both group, there was an age-associated decrease in the rate of activated oocytes. The decrease was more remarkable in male infertile group compared to the control group. CONCLUSIONS: Oocyte activation ability of spermatozoa appears to decrease with aging, in particular, among subfertile patients. This suggests that we need immediate assessment and appropriate intervention when handling male infertile patients.