MP65-01 STROMAL CELL DERIVED FACTOR-1 ACCELERATES RECOVERY OF CONTINENCE IN RAT MODEL OF VAGINAL DISTENSION INJURY

Abstract

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Female Incontinence: Therapy II1 Apr 2016MP65-01 STROMAL CELL DERIVED FACTOR-1 ACCELERATES RECOVERY OF CONTINENCE IN RAT MODEL OF VAGINAL DISTENSION INJURY Ahmad Khalifa, Amr Mahran, Michael Kavran, Juliana Woda, Marc Penn, and Adonis Hijaz Ahmad KhalifaAhmad Khalifa More articles by this author , Amr MahranAmr Mahran More articles by this author , Michael KavranMichael Kavran More articles by this author , Juliana WodaJuliana Woda More articles by this author , Marc PennMarc Penn More articles by this author , and Adonis HijazAdonis Hijaz More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.1212AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Vaginal distension (VD) in rats results in a reproducible injury to urethral continence mechanism that is recoverable in 2 weeks. VD injury has been shown to cause ischemia. Stromal cell derived factor-1 (SDF-1), is a known chemokine involved in angiogenesis and chemo attraction of stem cells into injury area. Previously in animal and human studies, delivery of non-viral human SDF-1 plasmid (pSDF-1), which expresses SDF-1 for 2-3 weeks, has shown safety, increased vessel density and improved patient status in ischemic heart and vascular diseases. In this experiment we examined the efficacy of pSDF-1 injection on continence recovery after VD & correlated the changes with histological changes. METHODS A total of 72 Sprague Dawley rats underwent VD and were randomly divided into 2 groups. Group 1 (42 rats) underwent SDF-1 injection followed by Leak point pressure (LPP) measurement at 4, 7, and 14 days (14 rats per time point). Group 2 (30 rats) were sham injected (5% dextrose) after VD and LPP was measured at same time points (10 rats per time point). VD was done as described with inflation of a 10 French modified Foley catheter intravaginal for 4 hours to create injury. SDF-1 and dextrose were injected peri-uretherally immediately after VD. Following LPP measurement, urogenital tissues were harvested for histological examination. Slides were stained with H&E and Masson’s trichrome. Vascularity and collagen content was determined using Adobe Photoshop and Image J software. Data analysis was done by IBM SPSS v.22. RESULTS As expected, the LPP decreased at 4 days in both groups. SDF-1 group exhibited a significant increase in LPP at 7 days compared to the sham (22.3 cmH2O vs 13.62 3 cmH2O, P value <0.001 respectively). The improvement in LPP continued in both groups at day 14, with the statistical difference approaching significance (36.38 cmH2O vs 25.82 cmH2O respectively, p=0.067). Histological evaluation and image analysis revealed no gross differences in the smooth and skeletal muscle thickness of the mid-urethra with no statistically significant difference in the collagen content and vascular density in the urethra at all-time points. CONCLUSIONS pSDF-1 plasmid injection at the time of VD accelerated the recovery of continence in the rat model of simulated birth trauma. The mechanism of improved recovery is not evident from the gross histological evaluation. Further research is needed to explain the mechanism of early improvement, possible hypothesizes are stem cell attraction and decrease in cellular injury or death. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e864 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Ahmad Khalifa More articles by this author Amr Mahran More articles by this author Michael Kavran More articles by this author Juliana Woda More articles by this author Marc Penn More articles by this author Adonis Hijaz More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...