PD35-05 HUMAN URINE-DERIVED STEM CELLS OR THEIR SECRETOME ALONE FACILITATE FUNCTIONAL RECOVERY IN A RAT MODEL OF STRESS URINARY INCONTINENCE

Abstract

You have accessJournal of UrologyStem Cell Research1 Apr 2016PD35-05 HUMAN URINE-DERIVED STEM CELLS OR THEIR SECRETOME ALONE FACILITATE FUNCTIONAL RECOVERY IN A RAT MODEL OF STRESS URINARY INCONTINENCE Christine Tran, Abhi Tangada, Hualin Yi, Brian Balog, Yuanyuan Zhang, and Margot Damaser Christine TranChristine Tran More articles by this author , Abhi TangadaAbhi Tangada More articles by this author , Hualin YiHualin Yi More articles by this author , Brian BalogBrian Balog More articles by this author , Yuanyuan ZhangYuanyuan Zhang More articles by this author , and Margot DamaserMargot Damaser More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.1077AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Stem cell based therapy has emerged as a promising alternative to current female stress urinary incontinence (SUI) treatments. Human urine-derived stem cells (USCs) have no risk from biopsy but have not previously been investigated for SUI. One therapeutic mechanism of action of stem cells is secretion of bioactive growth factors or ″secretome″ that drives the regulation of many physiological processes via paracrine actions. We hypothesized that USCs or their secretome alone would promote functional recovery in a rodent model of female SUI. METHODS Thirty two-female Sprague-Dawley rats were randomized into 3 groups and underwent vaginal distension (VD) followed by intraperitoneal delivery of USCs (VD + USC), VD and saline, or sham VD and saline 1 hour after injury. To investigate if factors secreted by USCs facilitate functional recovery after VD, concentrated conditioned media (CCM) was generated by incubating confluent USCs in serum-free media for 24 hours. Cultured supernatant was then extracted, washed, and concentrated to form CCM. Concentrated media (CM) was created by concentrating serum-free media without the influence of cells. Thirty rats randomized into 3 groups underwent VD and intraperitoneal delivery of CCM (VD + CCM), VD and CM, or sham VD and CM 1 hour after injury. One week after injury, treatment efficacy was assessed by measurement of leak point pressure (LPP), and histological and immunocytochemical analysis of the urethra. RESULTS After 1 week, LPP significantly increased after VD in rats treated with USCs or CCM, compared to animals that received saline, and was similar to sham VD animals. On semiquantitative histological analysis, there was no significant difference between both the striated and smooth muscle components of the urethral sphincter of VD + USC and VD + CCM animals compared to sham VD animals; VD + saline and VD + CM animals, however, demonstrated significant muscle fiber attenuation and disorganization. Additionally, elastin fibers in VD + USC and VD + CCM animals were long, thickened, and mostly oriented compared to the short, thin, and disoriented fibers in VD + saline and VD + CM animals. No implanted USCs were found in the region of urethra. CONCLUSIONS Intraperitoneal injection of USCs and their secretions facilitate recovery from SUI in a rat model, likely via paracrine effects. Furthermore, elastogenesis may play a role in recovery of urethral function. USCs represent an attractive, alternate stem cell source with no biopsy risk to target the underlying pathophysiology in female SUI. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e845 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Christine Tran More articles by this author Abhi Tangada More articles by this author Hualin Yi More articles by this author Brian Balog More articles by this author Yuanyuan Zhang More articles by this author Margot Damaser More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...