MP48-11 THE EFFECT OF MORPHINE AND ITS INHIBITORS ON RT112 BLADDER CANCER CELL GROWTH

Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology II1 Apr 2017MP48-11 THE EFFECT OF MORPHINE AND ITS INHIBITORS ON RT112 BLADDER CANCER CELL GROWTH Brian Birch, Bashir Lwaleed, Alan Cooper, Phil Harper, and Iliana Giatsidou Brian BirchBrian Birch More articles by this author , Bashir LwaleedBashir Lwaleed More articles by this author , Alan CooperAlan Cooper More articles by this author , Phil HarperPhil Harper More articles by this author , and Iliana GiatsidouIliana Giatsidou More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.1492AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Clinical studies suggest opioid use affects rates of metastasis in breast and prostate cancer. Results are variable and conflicting. This investigation tested the effects of morphine on bladder cancer cell growth in vitro and examined whether those effects can be reversed with receptor blockers. METHODS Cell lines: Adherent epithelial cell lines were used:RT-112 - Human urinary bladder transitional carcinoma cell lines, histological grade G2 (moderately differentiated). PNT2 - Normal (virally transformed) Human prostate epithelial cell line. These cells were grown using routine cell culture techniques supplemented with 10% foetal calf serum and antibiotics. Proliferation assay: The assay recorded residual viable biomass (RVB) 72 hours into exposure with the agent being studied RESULTS Doubling dilution of morphine produced a bell-shaped curve, with high (non-clinical)concentrations inhibiting growth, followed by stimulation between 100 and 1 microgram per ml (clinically relevant concentrations shown in Figure 1 green circle), reverting to control levels thereafter. The near-normal PNT-2 cell line showed minimal responsiveness to morphine Incubation with naloxone alone has little effect on residual biomass compared to controls Conversely, pre-incubating with a fixed concentration of Naloxone, then titrating morphine across the plate showed stimulation to decrease as the relative proportion of blocker rises (Figure 2) CONCLUSIONS Morphine in clinically relevant concentrations stimulated growth in the RT-112 transitional carcinoma cell line, as assessed by the MTT assay. This effect was minimal under the same conditions using the virally transformed normal prostate cell line (PNT2). Treating cells with the antagonists Naloxone or Naltrexone inhibited the action of morphine. The tentative implication is that using opioids in cancer patients despite their important role in pain relief may also tend to promote tumour growth. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e640-e641 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Brian Birch More articles by this author Bashir Lwaleed More articles by this author Alan Cooper More articles by this author Phil Harper More articles by this author Iliana Giatsidou More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...