Expression of Engraii-2 in different prostate cancer cell lines and normal human prostate epithelial cell line and its significance

Abstract

Objective To investigate the expression of Engrail-2 (EN2) and its significance in different prostate cancer cell lines and normal human prostate epithelial cell line.Methods The expression of EN2 was detected in prostate cancer cell lines (PC3,DU145,LNCAP) and normal prostate epithelial cell line RWPE-1 by using reverse transcription-polymerase chain reaction (RT-PCR),enzyme linked immunosorbent assay (ELISA) and immunofluorescence.Results The EN2 mRNA and protein expression levels in Pca cell lines ( LNCAP,PC3,DU145 ) were 1.65 ± 0.19/8.74 ± 0.34,1.08 ± 0.12/3.07 ±0.24,and 1.15 ±0.53/2.86± 0.26 respectively,which was significantly higher than in normal prostate epithelial cell line RWPE-1 (0.42 ± 0.11/0.86 ± 0.99 ) ( P ＜ 0.05 ).The EN2 level was higher in LNCAP cells than in PC3 and DU145 cells (P ＜ 0.05).Conclusion The expression of EN2 in 3 prostate cancer cell lines is higher than in RWPE-1 and EN2 protein can be secreted by prostate cancer cells.It is important for the further study on the EN2 potential roles in the occurrence and development of prostate carcinoma. Key words: Engrail-2; Homeobox protein; Prostate cancer