MP41-14 SERUM TRI-ANTENNARY N-GLYCAN IS OVER-EXPRESSED IN CASTRATION-RESISTANT PROSTATE CANCER

Abstract

You have accessJournal of UrologyProstate Cancer: Basic Research III1 Apr 2014MP41-14 SERUM TRI-ANTENNARY N-GLYCAN IS OVER-EXPRESSED IN CASTRATION-RESISTANT PROSTATE CANCER Yusuke Ishibashi, Tohru Yoneyama, Yuki Tobisawa, Shingo Hatakeyama, Takuya Koie, and Chikara Ohyama Yusuke IshibashiYusuke Ishibashi More articles by this author , Tohru YoneyamaTohru Yoneyama More articles by this author , Yuki TobisawaYuki Tobisawa More articles by this author , Shingo HatakeyamaShingo Hatakeyama More articles by this author , Takuya KoieTakuya Koie More articles by this author , and Chikara OhyamaChikara Ohyama More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.1231AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail Introduction and Objectives Cancer-associated glycan alteration is observed frequently during carcinogenesis. Recently, we have developed glycoblotting method which allows high-throughput, comprehensive and quantitative glycomics of human whole serum glycoproteins. Here we evaluated serum N-glycan biomarkers which correlate with castration-resistant status of prostate cancer PCa. Methods We performed N-glycome analysis from 80 Healthy volunteer (HLT), 289 benign prostatic hyperplasia (BPH), 272 PCa with non-hormone treatment, 45 PCa with hormone treatment and 41 castration-resistant PCa (CRPC) patients by glycoblotting method. Ten μL of whole serum was treated tyrpsin and peptide N-glycosidase F to release reducing N-glycan. The released total N-glycans in digested mixture were directly subjected to the glycoblotting using BlotGlyco beads. Recovered oligosaccharides were analyzed by MALDI-TOF/TOF mass spectrometer utilizing internal standard. Concentration of N-glycans in each patient was analyzed by logistic regression analysis and receiver operating characteristics (ROC) curve. Results Totally 70 kinds of N-glycans were detected. There were no significant differences in N-glycan profiles of HLT, BPH, early stage PCa and metastatic PCa with hormone treatment. We observed significant differences in N-glycan profiles between CRPC and other groups. Especially, tri-antennary N-glycan concentration in CRPC were significantly higher than HLT, BPH, early stage PCa, and metastaic PCa with hormone treatment. Conclusions These results indicate that serum tri-antennary N-glycan over-expression may be associated with castration-resistant status in PCa. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e456 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Yusuke Ishibashi More articles by this author Tohru Yoneyama More articles by this author Yuki Tobisawa More articles by this author Shingo Hatakeyama More articles by this author Takuya Koie More articles by this author Chikara Ohyama More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...