MP39-02 WHOLE-TRANSCRIPTOME PROFILING IN BLADDER PAIN SYNDROME/INTERSTITIAL CYSTITIS BY NEXT-GENERATION SEQUENCING: A DISTINCT GENOMIC LANDSCAPE BY THE PHENOTYPES

Abstract

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Interstitial Cystitis1 Apr 2018MP39-02 WHOLE-TRANSCRIPTOME PROFILING IN BLADDER PAIN SYNDROME/INTERSTITIAL CYSTITIS BY NEXT-GENERATION SEQUENCING: A DISTINCT GENOMIC LANDSCAPE BY THE PHENOTYPES Akiyama Yoshiyuki, Daichi Maeda, Hiroto Katoh, Aya Niimi, Akira Nomiya, Tetsuya Fujimura, Hiroshi Fukuhara, Haruki Kume, Yasuhiko Igawa, and Yukio Homma Akiyama YoshiyukiAkiyama Yoshiyuki More articles by this author , Daichi MaedaDaichi Maeda More articles by this author , Hiroto KatohHiroto Katoh More articles by this author , Aya NiimiAya Niimi More articles by this author , Akira NomiyaAkira Nomiya More articles by this author , Tetsuya FujimuraTetsuya Fujimura More articles by this author , Hiroshi FukuharaHiroshi Fukuhara More articles by this author , Haruki KumeHaruki Kume More articles by this author , Yasuhiko IgawaYasuhiko Igawa More articles by this author , and Yukio HommaYukio Homma More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.1249AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Bladder pain syndrome/interstitial cystitis (BPS/IC) comprises a diverse variety of clinical phenotypes. Among them, BPS/IC with Hunner lesions has been implied to be a distinct entity, histologically characterized by inflammatory infiltrates and urothelial denudation. In this study, we performed high-throughput sequencing-based whole transcriptome analysis to identify differentially expressed genes (DEGs) and characterize the genomic landscape in BPS/IC. METHODS A total of 54 urinary bladder biopsy samples were taken from 33 patients with BPS/IC with (12) (one each from the lesion and a non-lesion area; n = 24 in total) or without (21) Hunner lesions, and 9 non-IC patients without bladder symptoms and pathology, and subjected to whole RNA-sequencing. Hierarchical clustering and KEGG pathway analysis were performed for DEGs. RESULTS A total of 17,363 DEGs were identified among the groups (false discovery rate≤0.05). Hierarchical clustering analysis segregated samples into 2 distinct clusters. Cluster 1: BPS/IC with Hunner lesions. Cluster 2: BPS/IC without Hunner lesions and non-IC control (Fig. 1). KEGG pathway analysis revealed that DEGs upregulated in BPS/IC with Hunner lesions were significantly enriched in the T/B cell receptor signaling, chemokine signaling, Th17 cell differentiation, VEGF signaling, NF-κB signaling, NOD-like receptor signaling, Toll-like receptor signaling, inflammatory mediator regulation of TRP channels, and GAG degradation pathways, while those downregulated in BPS/IC with Hunner lesions were significantly related to the adherence/tight junction and estrogen signaling pathways (all P<0.05). On the other hand, DEGs in BPS without Hunner lesions were only 104 genes, for which KEGG pathway analysis could not find any significant biological pathways. CONCLUSIONS The results demonstrated a distinct genomic profile of BPS/IC with Hunner lesions. Urothelial deficiency, inflammatory/immune responses, aberrant vascularization, and abnormal estrogen signaling pathway may be involved in the pathophysiology of BPS/IC with Hunner lesions. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e510 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Akiyama Yoshiyuki More articles by this author Daichi Maeda More articles by this author Hiroto Katoh More articles by this author Aya Niimi More articles by this author Akira Nomiya More articles by this author Tetsuya Fujimura More articles by this author Hiroshi Fukuhara More articles by this author Haruki Kume More articles by this author Yasuhiko Igawa More articles by this author Yukio Homma More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...