MP38-18 ANDROGEN RECEPTOR SIGNALING IN CORPUS CAVERNOSUM IS SIMILAR IN MEN WITH SERUM TESTOSTERONE LEVELS ABOVE 200 ng/dL

Abstract

You have accessJournal of UrologyCME1 May 2022MP38-18 ANDROGEN RECEPTOR SIGNALING IN CORPUS CAVERNOSUM IS SIMILAR IN MEN WITH SERUM TESTOSTERONE LEVELS ABOVE 200 ng/dL Kajal Khodamoradi, Alexandra Dullea, Roei Golan, Jesse Ory, Himanshu Arora, and Ranjith Ramasamy Kajal KhodamoradiKajal Khodamoradi More articles by this author , Alexandra DulleaAlexandra Dullea More articles by this author , Roei GolanRoei Golan More articles by this author , Jesse OryJesse Ory More articles by this author , Himanshu AroraHimanshu Arora More articles by this author , and Ranjith RamasamyRanjith Ramasamy More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002592.18AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Testosterone (T) plays an important role in the proper development and maintenance of male reproductive tissues. The normal serum testosterone levels can vary between 300 to 1000 ng/dL. However, it is unclear whether androgen receptor signaling can vary based on serum T levels. What remains unknown is also whether there is a uniform serum T level above which androgen receptor signaling within the tissue remains similar (saturation hypothesis). Therefore, we evaluated whether varying serum levels of T alters androgen receptor (AR) signaling in human penile tissue. METHODS: We obtained human corpus cavernosum (CC) tissue biopsies during penile surgery (either for erectile dysfunction or Peyronie’s disease). We recorded their serum T levels one week prior to surgery. After mechanical dispersion of CC tissue with a homogenizer, the total protein was extracted using RIPA buffer, and quantitative detection of vascular endothelial growth factor (VEGF) was evaluated by western blot. RESULTS: The mean age of participants was 64±10 and the mean T level was 487.46ng/dL±377.20. The results of western blot showed that all corpus cavernosum samples in men with T >200ng/dL expressed similar levels of VEGF in men. However, men with a low serum T level (<200 ng/dL) had decreased expression of VEGF. The VEGF expression from men undergoing penile surgery for Peyronie's disease without erectile dysfunction was used as controls. CONCLUSIONS: Despite a wide range of serum T levels (200-1594 ng/dl), androgen receptor signaling was similar in penile tissue. This data suggests that the saturation value for penile androgen receptors appears to be around approximately 200ng/dL. Understanding androgen receptor saturation hypothesis provides important context necessary for appropriate prescribing of exogenous T to optimize patient outcomes. Source of Funding: This work was supported by Acerus Pharmaceuticals, National Institutes of Health Grant R01 DK130991 and Clinician Scientist Development Grant from the American Cancer Society to RR © 2022 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 207Issue Supplement 5May 2022Page: e625 Advertisement Copyright & Permissions© 2022 by American Urological Association Education and Research, Inc.MetricsAuthor Information Kajal Khodamoradi More articles by this author Alexandra Dullea More articles by this author Roei Golan More articles by this author Jesse Ory More articles by this author Himanshu Arora More articles by this author Ranjith Ramasamy More articles by this author Expand All Advertisement PDF DownloadLoading ...