MP36-08 GENOMIC AND IMMUNOHISTOCHEMICAL CHARACTERIZATION OF TYPES 1 AND 2 PAPILLARY RENAL CELL CARCINOMA: EVIDENCE FOR DISTINCT GROUPS OF DISEASES AND DIFFERENT PROGNOSTIC FEATURES

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You have accessJournal of UrologyKidney Cancer: Evalution/Staging III1 Apr 2014MP36-08 GENOMIC AND IMMUNOHISTOCHEMICAL CHARACTERIZATION OF TYPES 1 AND 2 PAPILLARY RENAL CELL CARCINOMA: EVIDENCE FOR DISTINCT GROUPS OF DISEASES AND DIFFERENT PROGNOSTIC FEATURES Alexandre Marsaud, Damien Ambrosetti, Matthieu Durand, Xavier Carpentier, Aurélie Floc'h, Yohann Rouscoff, Brannwel Tibi, Florence Dupre, Emmanuel Chamorey, Daniel Chevallier, Jean Amiel, Jean François Michiels, and Florence Pedeutour Alexandre MarsaudAlexandre Marsaud More articles by this author , Damien AmbrosettiDamien Ambrosetti More articles by this author , Matthieu DurandMatthieu Durand More articles by this author , Xavier CarpentierXavier Carpentier More articles by this author , Aurélie Floc'hAurélie Floc'h More articles by this author , Yohann RouscoffYohann Rouscoff More articles by this author , Brannwel TibiBrannwel Tibi More articles by this author , Florence DupreFlorence Dupre More articles by this author , Emmanuel ChamoreyEmmanuel Chamorey More articles by this author , Daniel ChevallierDaniel Chevallier More articles by this author , Jean AmielJean Amiel More articles by this author , Jean François MichielsJean François Michiels More articles by this author , and Florence PedeutourFlorence Pedeutour More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.1072AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Papillary renal cell carcinomas (pRCC) are the second most common type of RCC. The World Health Organization classification of renal tumors distinguishes two types of pRCC based on morphological microscopic, immunohistochemical and genetic features. However, the accurate diagnosis of these tumors is often challenging, and their evolution still remains indefinite. Our aim was to identify molecular biomarkers useful for diagnostic workup and prognosis assessment in pRCC. METHODS Among a series of renal cell tumors in patients who underwent nephrectomy at a cancer reference center between 2005 and 2012, 32 consecutive pRCC cases were selected. Immunohistochemical analyses and genetic investigations consisting of karyotype, fluorescence in situ hybridization (FISH) and comparative genomic hybridization on arrays (array-CGH) were carried out on the specimens. RESULTS Out of 32 tumors, 15 were of type 1 and 17 of type 2. Cytokeratin-7 was expressed in 100% and 68% of type 1 and 2, respectively. CD 10, Vimentin, p504S and TFE3 were of similar expression in both types (70%, 100%, 100% and 0% respectively). Association of gains of chromosomes 7 and 17 (and loss of chromosome Y) were reported in all 15 cases of type 1-pRCC but only in 64 % of type 2-pRCC. We identified three different genomic subgroups in type 2-pRCC: -i) similar genetic profiles to type1-pRCC; -ii) partial common genetic features; -iii) genetic profiles different from type1-pRCC. Our results pointed out that one third of tumors diagnosed as type2-pRCC may be unrelated to type1-pRCC despite papillary architecture. We observed significant differences in survival correlated to some specific genetic alterations (cf figure 1). In contrast, no significant difference was detected regarding the gender, papillary subtype and immunochemistry. CONCLUSIONS Our results showed that type2-pRCC did not constitute a homogeneous group. While some type2-pRCCs were genetically related to type1-pRCCs, some others may have established distinct diseases. Moreover, we observed significant difference in survival correlated to genetic alterations. A longer cohort is expected to confirm these results likely leading to personalized treatments based on genetic profiles. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e381-e382 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Alexandre Marsaud More articles by this author Damien Ambrosetti More articles by this author Matthieu Durand More articles by this author Xavier Carpentier More articles by this author Aurélie Floc'h More articles by this author Yohann Rouscoff More articles by this author Brannwel Tibi More articles by this author Florence Dupre More articles by this author Emmanuel Chamorey More articles by this author Daniel Chevallier More articles by this author Jean Amiel More articles by this author Jean François Michiels More articles by this author Florence Pedeutour More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...