MP35-20 A NEW URINARY CATHETER DESIGN REDUCES IN VITRO BIOFILM FORMATION BY INFLUENCING HYDRODYNAMICS

Abstract

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Prostate & Genitalia (MP35)1 Sep 2021MP35-20 A NEW URINARY CATHETER DESIGN REDUCES IN VITRO BIOFILM FORMATION BY INFLUENCING HYDRODYNAMICS Andrei Ionescu, Eugenio Brambilla, Maria Chiara Sighinolfi, and Roberto Mattina Andrei IonescuAndrei Ionescu More articles by this author , Eugenio BrambillaEugenio Brambilla More articles by this author , Maria Chiara SighinolfiMaria Chiara Sighinolfi More articles by this author , and Roberto MattinaRoberto Mattina More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002044.20AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: To evaluate the performance of a new catheter design based on different hydrodynamics – aiming to reduce the development of biofilm, and compare it with conventional Foley catheter (FC, control). METHODS: The new proposed design catheter (NPD, test) is a modification of the FC: it is based on an asymmetrical positioning of the balloon and additional drainage holes allowing for continuous urine drainage and complete voiding of the bladder. A first experiment was accomplished to assess the drainage capability. A second experiment was performed using a bioreactor with a setup simulating the bladder and using the tested catheter as a flow-through system. Biofilm formation of five bacterial species associated with CAUTI was determined after 24 h incubation using MTT assay. Morphological evaluation was performed using SEM. In vitro determination of residual fluid, quantitative and morphological data about biofilm formation on the intravesical and intraluminal part of the tested catheters were assessed. RESULTS: Residual fluid in FC (5.60±0.43 ml) was significantly higher than in NPD (0.2±0.03 ml). NPD showed significantly lower biofilm formation (p<0.0001) than FC. Catheter design had a different effect on biofilm formation depending on the tested strain. The intraluminal amount of biomass was significantly lower than the intravesical one in both catheters (p<0.0001). Multilayered biofilms completely covering FC surfaces were seen for all tested strains, while NPD showed reduced biofilm formation. CONCLUSIONS: Modifications of a catheter’s hydrodynamic characteristics can significantly reduce bacterial colonization. Integrated design approaches combining chemical, mechanical, and topographical elements can help in lowering CAUTI occurrence. Source of Funding: none © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e635-e635 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Andrei Ionescu More articles by this author Eugenio Brambilla More articles by this author Maria Chiara Sighinolfi More articles by this author Roberto Mattina More articles by this author Expand All Advertisement Loading ...