PD52-03 EVALUATION OF PROTECTION AGAINST GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA IN A NOVEL IN VITRO BIOFILM MODEL OF PENILE PROSTHESIS

Abstract

You have accessJournal of UrologyCME1 May 2022PD52-03 EVALUATION OF PROTECTION AGAINST GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA IN A NOVEL IN VITRO BIOFILM MODEL OF PENILE PROSTHESIS Manish Narasimman, Gregory Plano, Jesse Ory, Sara Schesser Bartra, and Ranjith Ramasamy Manish NarasimmanManish Narasimman More articles by this author , Gregory PlanoGregory Plano More articles by this author , Jesse OryJesse Ory More articles by this author , Sara Schesser BartraSara Schesser Bartra More articles by this author , and Ranjith RamasamyRanjith Ramasamy More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002629.03AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Late infection, thought to be due to biofilm formation, remains a feared complication after inflatable penile prosthesis (IPP) insertion. Our in vitro model previously showed S. epidermidis, S. aureus, P. aeruginosa, and K. pneumoniae can form significant biofilm on IPPs. P. aeruginosa showed the strongest predilection to grow biofilm. Understanding and preventing this biofilm formation could be a breakthrough in reducing IPP infections. We used our in vitro model to compare growth of different bacterial biofilms on IPPs with and without antibiotics and compare efficacy of antibiotic treated Coloplast Titan® and AMS 700 InhibiZone™ against biofilm formation. METHODS: Sterile IPPs were cut into rings. Coloplast rings were dipped in 10 ml of a 10 mg/ml Rifampin, 1 mg/ml Gentamicin, and deionized water solution and dried. Coloplast and AMS rings were incubated in S. epidermidis, S. aureus, P. aeruginosa, or K. pneumoniae cultures in tryptic soy broth (TSB) for a 2 h attachment period, followed by only TSB for 120 h. We utilized a published and validated method to measure biofilm mass. Rings were fixed with ethanol and biofilm formation measured by spectrophotometer (OD570) after crystal violet staining. RESULTS: Of the bacteria studied, only P. aeruginosa formed significant biofilm on antibiotic treated Coloplast Titan® and AMS 700 InhibiZone™ implant rings. In direct comparison of the implants, no significant difference was found in biofilm formation by P. aeruginosa. CONCLUSIONS: Our novel in vitro model of biofilm formation of IPPs evaluated the effect of a gentamicin/rifampin antibiotic dip on Coloplast Titan® and the anti-infective capacity of minocycline/rifampin precoated AMS 700 InhibiZone™ against various bacteria. Our findings suggest P. aeruginosa can form a significant quantity of biofilm on IPPs even with antibiotic treatments. However, both treated IPPs appear to provide effective coverage against biofilm formation by S. epidermidis, S. aureus, and K. pneumoniae. No significant difference was found between the IPPs in protection against P. aeruginosa. P. aeruginosa should continue to be a specific target of investigation and antibiotic strategies potentially modified to increase coverage against the organism to reduce delayed post-operative IPP infections. Source of Funding: This work was supported by National Institutes of Health Grant R01 DK130991 and Clinician Scientist Development Grant from the American Cancer Society to Ranjith Ramasamy. This work was also supported by the AUA Summer Medical Student Fellowship Award to Manish Narasimman © 2022 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 207Issue Supplement 5May 2022Page: e905 Advertisement Copyright & Permissions© 2022 by American Urological Association Education and Research, Inc.MetricsAuthor Information Manish Narasimman More articles by this author Gregory Plano More articles by this author Jesse Ory More articles by this author Sara Schesser Bartra More articles by this author Ranjith Ramasamy More articles by this author Expand All Advertisement PDF DownloadLoading ...