MP28-02 PHYSIOLOGICAL EVIDENCE OF DNA DAMAGE BY CARCINOGENS KNOWN TO BE PRESENT IN CHARRED AND PROCESSED MEATS (PHIP DNA ADDUCTS), IN A SMALL COHORT OF PROSTATE CANCER PATIENTS.

Abstract

You have accessJournal of UrologyProstate Cancer: Markers I1 Apr 2017MP28-02 PHYSIOLOGICAL EVIDENCE OF DNA DAMAGE BY CARCINOGENS KNOWN TO BE PRESENT IN CHARRED AND PROCESSED MEATS (PHIP DNA ADDUCTS), IN A SMALL COHORT OF PROSTATE CANCER PATIENTS. Christopher Weight, Shun Xiao, Jingshu Guo, Byeong Hwa Yun, Badrinath Konety, Peter Villalta, Resha Tejpaul, Suprita Krishna, Paari Murugan, and Robert Turesky Christopher WeightChristopher Weight More articles by this author , Shun XiaoShun Xiao More articles by this author , Jingshu GuoJingshu Guo More articles by this author , Byeong Hwa YunByeong Hwa Yun More articles by this author , Badrinath KonetyBadrinath Konety More articles by this author , Peter VillaltaPeter Villalta More articles by this author , Resha TejpaulResha Tejpaul More articles by this author , Suprita KrishnaSuprita Krishna More articles by this author , Paari MuruganPaari Murugan More articles by this author , and Robert TureskyRobert Turesky More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.815AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Epidemiologic studies have reported an association between frequent consumption of well-done cooked meats and prostate cancer (PC) risk. Charred red meat and cooked processed meats are known to contain heterocyclic aromatic amine (HAA) carcinogens, such as 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) the most mass abundant HAA, and are linked to PC development in a rodent model. However, unambiguous physiochemical markers of DNA damage from these meat-derived carcinogens have not been identified in human samples to support the paradigm of HAA induced human prostate carcinogenesis. METHODS Thirty-five men with biopsy proven intermediate to high-risk PC underwent radical prostatectomy at University of Minnesota from Dec 2015-Aug 2016. After prostatectomy, both tumor bearing tissue and non-tumor bearing adjacent fresh tissue was analyzed for DNA adducts using a highly sensitive nano-LC-Orbitrap mass spectrometry method. We also analyzed formalin fixed paraffin embedded (FFPE) tissues from each patient. RESULTS Median age of the men with PC was 65 (range 45-78). Pathology demonstrated the following Gleason Scores (GS) and pathologic staging: GS= 6 in 1 patient (2.8%), GS=7 in 28 patients (80%) and GS=8-10 in 6 patients (17%) and 16 men (46%) were stage 2 and 19 men were stage 3 (54%). The PhIP DNA adduct was identified in 11 out of 35 patients, at levels ranging from 2 to 120 adducts per 109 nucleotides. PhIP DNA adducts also were recovered quantitatively from FFPE tissues. CONCLUSIONS Our data provide support to the epidemiological observations implicating PhIP as a DNA damaging agent that may contribute to the etiology of PC in humans. FFPE tissues can be used as a tissue source in DNA-adduct biomarker research using our mass spectrometry method. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e338 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Christopher Weight More articles by this author Shun Xiao More articles by this author Jingshu Guo More articles by this author Byeong Hwa Yun More articles by this author Badrinath Konety More articles by this author Peter Villalta More articles by this author Resha Tejpaul More articles by this author Suprita Krishna More articles by this author Paari Murugan More articles by this author Robert Turesky More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...