MP16-08 SEARCH FOR MICROORGANISMS IN MEN WITH UROLOGIC CHRONIC PELVIC PAIN SYNDROME: A CULTURE-INDEPENDENT ANALYSIS OF CASES AND CONTROLS ENROLLED IN THE TRANS-MAPP EPIDEMIOLOGY/PHENOTYPING (EP) STUDY

Abstract

You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Prostate & Genitalia1 Apr 2014MP16-08 SEARCH FOR MICROORGANISMS IN MEN WITH UROLOGIC CHRONIC PELVIC PAIN SYNDROME: A CULTURE-INDEPENDENT ANALYSIS OF CASES AND CONTROLS ENROLLED IN THE TRANS-MAPP EPIDEMIOLOGY/PHENOTYPING (EP) STUDY J. Curtis Nickel, Alisa Stephens, Jun Chen, Richard Landis, Christopher Mullins, Garth Ehrlich, and The MAPP Research Network J. Curtis NickelJ. Curtis Nickel More articles by this author , Alisa StephensAlisa Stephens More articles by this author , Jun ChenJun Chen More articles by this author , Richard LandisRichard Landis More articles by this author , Christopher MullinsChristopher Mullins More articles by this author , Garth EhrlichGarth Ehrlich More articles by this author , and The MAPP Research Network More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.581AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES We hypothesized that differences in lower urinary tract microbial populations between male UCPPS patients and normal subjects may be implicated in the etiology of male UCPPS. We used novel state-of-the-art culture-independent methodology to compare the microbiota of the lower urinary tract in men with UCPPS and controls enrolled in the NIH trans-MAPP EP study. METHODS Each participant (cases and non-UCPPS controls) were asked to provide initial (VB1), midstream (VB2) and post-prostatic massage (VB3) urine specimen. Specimens were analyzed with Ibis T-5000 Universal Biosensor technology which employs a polymerase chain reaction – electron spray ionization – time-of-flight – mass spectoscopic- based system to provide comprehensive identification of all bacterial species. Differences between UCPPS patients and controls in the presence of species or the number of different species within a higher level group were tested using PERMANOVA, and Fisher’s Exact test. All tests were conducted at the species, genus,and gram-stain level. RESULTS 417 male subjects (191 cases; 226 controls) were enrolled in the trans-MAPP-EP study. Baseline VB1 and VB2 urine specimens were obtained from 110 UCPPS cases and 115 matched controls (subjects with no UCPPS symptoms). VB3 samples were provided by 67 CP/CPPS and 62 controls. A total of 78, 73 and 54 species (42, 39 and 27 genera) were detected in VB1, VB2 and VB3 respectively. Mean (SD) VB1, VB2 and VB3 species count per person was 1.62 (1.28), 1.38 (1.36) and 1.33(1.24) for cases and 1.75(1.32), 1.23(1.15) and 1.56 (0.97) for controls respectively. Overall species and genus composition significantly differed between UCPPS patients and controls in VB1 only (p=0.008 species level, p=0.011 genus level, p=0.079 Gram-stain level) with overall differences driven by B. cenocepacia (RR=3.34, p=0.009), P. acnes (RR 0.38, p=0.01), and S. capitis/capare (RR=0.17, p=0.008). These differences (and similar trends at genus level) remained significant after adjustment for multiple comparisons. No significant differences were observed at the Gram-stain level in VB1 or at any level in VB2 or VB3. CONCLUSIONS While our results make it difficult to implicate the minor differences in lower urinary tract microbiota (VB1 only) in patients vs controls for a role in the etiology and pathogenesis of UCPPS in males, further work is required to determine if microbial composition or fluctuations influence symptom patterns over time. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e156 Advertisement Copyright & Permissions© 2014MetricsAuthor Information J. Curtis Nickel More articles by this author Alisa Stephens More articles by this author Jun Chen More articles by this author Richard Landis More articles by this author Christopher Mullins More articles by this author Garth Ehrlich More articles by this author The MAPP Research Network More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...