MP82-18 SHARED ALTERATIONS IN URINARY BACTERIAL COMMUNITIES IN PATIENTS WITH INTERSTITIAL CYSTITIS AND OVERACTIVE BLADDER

Abstract

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology II1 Apr 2017MP82-18 SHARED ALTERATIONS IN URINARY BACTERIAL COMMUNITIES IN PATIENTS WITH INTERSTITIAL CYSTITIS AND OVERACTIVE BLADDER A. Lenore Ackerman, Jie Tang, Karyn Eilber, Jayoung Kim, J. Curtis Nickel, Garth Ehrlich, James Ackerman, David Underhill, Michael Freeman, Jennifer Anger, and MAPP Research Network A. Lenore AckermanA. Lenore Ackerman More articles by this author , Jie TangJie Tang More articles by this author , Karyn EilberKaryn Eilber More articles by this author , Jayoung KimJayoung Kim More articles by this author , J. Curtis NickelJ. Curtis Nickel More articles by this author , Garth EhrlichGarth Ehrlich More articles by this author , James AckermanJames Ackerman More articles by this author , David UnderhillDavid Underhill More articles by this author , Michael FreemanMichael Freeman More articles by this author , Jennifer AngerJennifer Anger More articles by this author , and MAPP Research NetworkMAPP Research Network More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.2565AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Despite historical assumptions that urine is sterile, accumulating research demonstrates that adult women have diverse bacterial ecosystems resident in the urine. Changes in these communities are associated with benign lower urinary tract conditions, such as overactive bladder (OAB) and interstitial cystitis/painful bladder syndrome (IC/PBS). As there is significant symptomatic overlap between these two conditions, we sought to characterize changes in the urinary microbiome in a spectrum of patients with these bladder hypersensitivity syndromes to clarify potential shared pathophysiologic mechanisms. METHODS We used high-throughput 16S rRNA gene sequencing using an Illumina MiSeq next generation sequencing platform to identify bacterial DNA isolated from catheterized urine specimens obtained from asymptomatic controls (n=14) and subjects with IC/PBS (n=13) or OAB (n=17). After classifying bacterial taxa via alignment to multiple bacterial 16S sequence databases, urinary communities were compared at a population level between experimental groups. RESULTS Women with OAB or IC/PBS demonstrated decreased bacterial diversity at the genus level in comparison to controls, but this difference was more profound in IC/PBS (Figure). Several unique genera were altered in these conditions in comparison to controls. Changes in the levels of Burkholderia, a genus recently identified in association with chronic pelvic pain in males (Nickel et al., 2015), were associated with disease in our population, with a stronger association with OAB than IC/PBS. In addition, alterations in Lactobacillus species were also prevalent in both conditions, but more pronounced in patients with IC/PBS. CONCLUSIONS Bacterial communities resident within the lower urinary tract are altered in the presence of lower urinary tract symptoms. The microbiomes of urine from patients with OAB and IC/PBS, however, were similar when analyzed in parallel, varying more with symptom severity than with diagnosis. Regardless of diagnosis, greater symptom severity inversely correlated with bacterial diversity. These results support recent propositions that OAB and IC/PBS may represent points on a spectrum of disease sharing a similar pathophysiology. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e1104 Advertisement Copyright & Permissions© 2017MetricsAuthor Information A. Lenore Ackerman More articles by this author Jie Tang More articles by this author Karyn Eilber More articles by this author Jayoung Kim More articles by this author J. Curtis Nickel More articles by this author Garth Ehrlich More articles by this author James Ackerman More articles by this author David Underhill More articles by this author Michael Freeman More articles by this author Jennifer Anger More articles by this author MAPP Research Network More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...