MP10-19 MATURE RANDALL’S PLAQUE CONTAINS CELL NUCLEI: OBSERVATIONS USING HIGH-RESOLUTION MICROSCOPY

Abstract

You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology II (MP10)1 Apr 2020MP10-19 MATURE RANDALL’S PLAQUE CONTAINS CELL NUCLEI: OBSERVATIONS USING HIGH-RESOLUTION MICROSCOPY Victor Hugo Canela*, Sharon B. Bledsoe, Glenn S. Gerber, Elaine M. Worcester, James E. Lingeman, Tarek M. El-Achkar, and James C. Williams Victor Hugo Canela*Victor Hugo Canela* More articles by this author , Sharon B. BledsoeSharon B. Bledsoe More articles by this author , Glenn S. GerberGlenn S. Gerber More articles by this author , Elaine M. WorcesterElaine M. Worcester More articles by this author , James E. LingemanJames E. Lingeman More articles by this author , Tarek M. El-AchkarTarek M. El-Achkar More articles by this author , and James C. WilliamsJames C. Williams More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000830.019AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The formation of CaOx stones on Randall’s plaque is a common phenomenon (perhaps 25% of all stones), and stones that have grown on Randall’s plaque can be easily identified using simple microscopy, yet this mechanism of stone formation is still poorly understood. The objective of this study was to identify differences in the structure and composition of Randall’s plaque that differentiate it from the CaOx overgrowth region and to shed light on possible mechanisms for plaque formation. METHODS: Randall’s plaque stones were mounted in gel along with fiducial markers and scanned by micro CT to document orientation. Following demineralization, the stone was embedded in paraffin and sectioned for histology and fluorescence imaging. RESULTS: Demineralized and sectioned Randall’s plaque displayed the same autofluorescence in the far-blue region that we have previously documented in mineralized plaque. Microscopic analysis demonstrates that the plaque autofluoresces in the far-blue range as opposed to the CaOx overgrowth which appears mostly with green or yellow autofluorescence. Further, high-resolution imaging of the sectioned plaque showed that this blue autofluorescence was present throughout the demineralized plaque, but that its intensity was diminished in oval regions of the right size to be nuclei of cells. Subsequent staining of sections with the DNA marker, 7-aminoactinomycin D (7-AAD), confirmed the presence of nuclei within the demineralized Randall’s plaque. CONCLUSIONS: This is the first demonstration of the presence of cells within areas of mature Randall’s plaque. The nature of cells and their role in plaque formation are yet to be determined. Our data suggest that these cells contain ordinary nuclear morphology and are preserved within the calcium phosphate plaque. The presence of nuclei in the mature plaque means that apoptosis and/or necrosis is not part of the normal process of the formation of fully mineralized Randall’s plaque. Furthermore, the nuclei were exclusively detected in the plaque area and not in the CaOx overgrowth. Proteomic analysis in the different regions along with the interface region will provide invaluable information in understanding the growth of CaOx on Randall’s plaque. Source of Funding: NIDDK P01 56788 © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e133-e133 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Victor Hugo Canela* More articles by this author Sharon B. Bledsoe More articles by this author Glenn S. Gerber More articles by this author Elaine M. Worcester More articles by this author James E. Lingeman More articles by this author Tarek M. El-Achkar More articles by this author James C. Williams More articles by this author Expand All Advertisement PDF downloadLoading ...