MP1-19 ACCUMULATION OF VERSICAN, AN EXTRACELLULAR MATRIX COMPONENT, IN PELVIC FLOOR TISSUES IN A RAT MODEL OF SIMULATED CHILDBIRTH INJURY

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research I1 Apr 2014MP1-19 ACCUMULATION OF VERSICAN, AN EXTRACELLULAR MATRIX COMPONENT, IN PELVIC FLOOR TISSUES IN A RAT MODEL OF SIMULATED CHILDBIRTH INJURY Chong Choe, Marika Bogdani, Thomas Wight, and Una Lee Chong ChoeChong Choe More articles by this author , Marika BogdaniMarika Bogdani More articles by this author , Thomas WightThomas Wight More articles by this author , and Una LeeUna Lee More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.117AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Abnormal extracellular matrix (ECM) has been correlated with stress urinary incontinence (SUI). Most ECM studies have focused on alterations in collagen and elastin. However, other ECM components such as proteoglycans (PG) play an important role in the remodeling of collagen and elastin. Versican (VC) is a PG that plays a key regulatory role in maintaining elastic fiber networks. VC, however, has not yet been investigated in SUI. Vaginal distension (VD) is a well accepted rodent model of simulated childbirth injury used to study SUI. The aim was to investigate the effects of VD in rats on the distribution of VC on urethral, para-urethral, and anterior vaginal wall tissues. METHODS Nulliparous Sprague-Dawley female rats underwent VD or sham. Pelvic floor tissues were harvested for immunohistochemical staining for VC at days 1, 4, and 21. Tissues were collected from 6 rats per group at each time point. RESULTS Sham injured rats had low VC staining around the urethra and minimal to no VC staining in the para-urethral and vaginal lamina propria (LP) at all time points (fig 1, left). At day 1, VD rats had increased VC staining in the urethra. By day 4, VD rats had increased staining in the urethra, para-urethral tissues, and vaginal LP (fig 1, right). At day 21, VD rats had similar persistent areas of VC staining as the day 4 VD rats. CONCLUSIONS This study demonstrates a distinct pattern of increased versican accumulation in the urethra, para-urethral tissues, and vaginal lamina propria after vaginal distension. Since extracellular matrix has been shown to be abnormal in SUI, studying the patterns of proteoglycan accumulation may lead to an understanding of why elastic fibers are altered in pelvic floor tissues in patients with SUI. Versican accumulation warrants further study to better understand alterations in tissue support and function for the future development of potential targeted therapies. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e8 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Chong Choe More articles by this author Marika Bogdani More articles by this author Thomas Wight More articles by this author Una Lee More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...