MP1-13 PUBIC SYMPHYSIS LENGTH IS CORRELATED WITH PELVIC ORGAN PROLAPSE IN LYSYL OXIDASE LIKE-1 KNOCKOUT MICE

Abstract

Listen

Translate article

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research I1 Apr 2014MP1-13 PUBIC SYMPHYSIS LENGTH IS CORRELATED WITH PELVIC ORGAN PROLAPSE IN LYSYL OXIDASE LIKE-1 KNOCKOUT MICE Ali Borazjani, Bruna Couri, Brian Balog, and Margot Damaser Ali BorazjaniAli Borazjani More articles by this author , Bruna CouriBruna Couri More articles by this author , Brian BalogBrian Balog More articles by this author , and Margot DamaserMargot Damaser More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.111AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Changes in extracellular matrix (ECM) metabolism have been observed in women with pelvic organ prolapse (POP). Similar to women, parity is the leading risk factor for POP in lysyl oxidase like-1 knockout (LOXL1 KO) mice and ∼50% of parous LOXL1 KO mice develop POP by middle age. In mammals, the pubic symphysis (PS) undergoes significant elongation during pregnancy in preparation for delivery. This alters the bony pelvic geometry may lead to stretch-induced remodeling of the pelvic soft-tissues. The goal of this project was to assess changes in PS morphometry and vaginal weight in LOXL1 KO mice. METHODS Vaginal weight and calibrated images (35x) of intact PS were taken in LOXL1 KO (n=44) and WT (n=34) primiparous (25 day, 7wk, and 12 wk postpartum) and age-matched nulliparous mice. Odds ratio (OR) and 95% confidence interval (CI) for determining the likelihood of a measure above the 75th percentile for each time point were calculated. Spearman Rank Order Correlation was used to determine associations between PS length and vaginal weight. An additional group consisting of KO mice with POP (n=4) and age/parity-matched WT mice without POP (n=3) were dissected for PS and vaginal measurements. Student’s t-test was used to compare means between groups, and correlations were calculated to determine the association between prolapse and PS length. RESULTS Primiparous KO mice were 12 times more likely to have an elongated PS compared to WT mice (OR: 12.05, 95% CI: 1.43-101.5, p=0.017). Additionally, PS length was significantly correlated to vaginal weight (correlation coefficient [CC]: 0.352, p=0.002). KO mice at the earlier 2 postpartum time points had lower body weights and vaginal weights than WT mice. The correlation between PS length and vaginal weight persisted and strengthened after normalizing vaginal weight to body weight (CC: 0.394, p<0.001). There was no difference in PS length between KO and WT mice at any time point in nulliparous mice. PS length in mice with POP was significantly longer compared to mice without POP (2.86±0.35mm vs 0.38±0.12mm, p=0.003), and PS length was strongly correlated with prolapse (CC: 0.866, p<0.001). CONCLUSIONS PS length in mice with POP was over 7-fold higher than mice without POP. Mice prone to developing POP (i.e. parous LOXL1 KO) were more likely to have PS elongation compared to WT mice following pregnancy and parturition. This finding was dependent on parity, given that there was no difference in PS lengths between nulliparous animals. This study supports the need for future studies investigating the correlation between PS length and POP in women. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e6 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Ali Borazjani More articles by this author Bruna Couri More articles by this author Brian Balog More articles by this author Margot Damaser More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...