MP09-03 HYDROGEN SULFIDE CAN FUNCTION AS AN ENDOGENOUS RELAXATION FACTOR IN THE RAT BLADDER AND PROSTATE

Abstract

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology & Pharmacology I1 Apr 2018MP09-03 HYDROGEN SULFIDE CAN FUNCTION AS AN ENDOGENOUS RELAXATION FACTOR IN THE RAT BLADDER AND PROSTATE Takahiro Shimizu, Suo Zou, Shogo Shimizu, Youichirou Higashi, Hideaki Ono, Kumiko Nakamura, Takaaki Aratake, Masaki Yamamoto, Tomoya Hamada, Yoshiki Nagao, Masashi Honda, and Motoaki Saito Takahiro ShimizuTakahiro Shimizu More articles by this author , Suo ZouSuo Zou More articles by this author , Shogo ShimizuShogo Shimizu More articles by this author , Youichirou HigashiYouichirou Higashi More articles by this author , Hideaki OnoHideaki Ono More articles by this author , Kumiko NakamuraKumiko Nakamura More articles by this author , Takaaki AratakeTakaaki Aratake More articles by this author , Masaki YamamotoMasaki Yamamoto More articles by this author , Tomoya HamadaTomoya Hamada More articles by this author , Yoshiki NagaoYoshiki Nagao More articles by this author , Masashi HondaMasashi Honda More articles by this author , and Motoaki SaitoMotoaki Saito More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.329AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES We previously reported that hydrogen sulfide (H2S), an endogenous gasotransmitter, induced relaxation on rat bladder and prostate strips (AUA2015, MP21-01). In this study, we examined (1) endogenous H2S contents and expression levels of 5 enzymes of H2S synthesis, cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), 3-mercaptopyruvate sulftransferase (MPST), cysteine aminotransferase (CAT) and D-amino acid oxidase (DAO) in the rat bladder and prostate, and (2) effects of intravesical instillation of GYY4137 (GYY), an H2S donor, on micturition in rats. METHODS (1) Bladder dome and trigone (BL-D and BL-T), and dorsolateral and ventral prostate (PR-D and PR-V) were prepared from male Wistar rats (300-400 g). H2S contents were measured by methylene blue method. Expression levels of 5 enzymes were examined by quantitative real-time PCR, western blotting and immunohistochemistry. (2) In urethane (0.8 g/kg, ip)-anesthetized male Wistar rats (300-400 g), a catheter was inserted into the bladder in order to instill reagents (2.4 ml/h) and to measure intravesical pressure. After detecting 4-5 micturition reflexes induced by saline instillation, GYY solution (10-8, 10-7 and 10-6 M, in turn) or vehicle was instilled. RESULTS (1) H2S was detected in both bladder and prostate and the content was the highest in the BL-T. In both gene and protein levels, MPST and CAT were detected in the bladder and prostate and CBS was only detected in PR-D and PR-V, while CSE and DAO were not detected in these 4 tissues. In the PR-D and PR-V, immunoreactivities of CBS, MPST and CAT were detected in the glandular epithelium (Fig. 1). In the BL-D and BL-T, MPST and CAT were detected in the urothelium and smooth muscle layers, while CBS was not detected (Fig. 1). (2) Intravesical instillation of GYY significantly prolonged intercontraction intervals compared to the vehicle-treated group (Fig. 2) without altering maximal voiding pressure. CONCLUSIONS H2S can function as an endogenous relaxation factor in the rat bladder and prostate. Therefore, endogenous H2S might open new avenues of therapeutic interventions for lower urinary tract dysfunction such as overactive bladder and benign prostatic hyperplasia. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e106 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Takahiro Shimizu More articles by this author Suo Zou More articles by this author Shogo Shimizu More articles by this author Youichirou Higashi More articles by this author Hideaki Ono More articles by this author Kumiko Nakamura More articles by this author Takaaki Aratake More articles by this author Masaki Yamamoto More articles by this author Tomoya Hamada More articles by this author Yoshiki Nagao More articles by this author Masashi Honda More articles by this author Motoaki Saito More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...