MP28-10 COMPARISON OF THE HYDROGEN SULFIDE-INDUCED RELAXATION OF THE BLADDER BETWEEN HYPERTENSIVE AND NORMOTENSIVE RATS

Abstract

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology & Pharmacology I (MP28)1 Apr 2020MP28-10 COMPARISON OF THE HYDROGEN SULFIDE-INDUCED RELAXATION OF THE BLADDER BETWEEN HYPERTENSIVE AND NORMOTENSIVE RATS Suo Zou*, Takahiro Shimizu, Shogo Shimizu, Youichirou Higashi, Takaaki Aratake, Masaki Yamamoto, Tomoya Hamada, Yoshiki Nagao, Masashi Honda, and Motoaki Saito Suo Zou*Suo Zou* More articles by this author , Takahiro ShimizuTakahiro Shimizu More articles by this author , Shogo ShimizuShogo Shimizu More articles by this author , Youichirou HigashiYouichirou Higashi More articles by this author , Takaaki AratakeTakaaki Aratake More articles by this author , Masaki YamamotoMasaki Yamamoto More articles by this author , Tomoya HamadaTomoya Hamada More articles by this author , Yoshiki NagaoYoshiki Nagao More articles by this author , Masashi HondaMasashi Honda More articles by this author , and Motoaki SaitoMotoaki Saito More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000867.010AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: We recently reported that hydrogen sulfide (H2S), an endogenous gasotransmitter, is a possible relaxation factor in the rat bladder. Because it has shown that bladder dysfunctions can be developed by chronic hypertension in spontaneously hypertensive rats (SHRs), we compared effects of NaHS and GYY4137 (H2S donors) on the bladder contractility and the micturition reflex, and the endogenous H2S system in the bladder between SHRs and normotensive Wistar rats (Wistars). METHODS: Eighteen-week-old male SHRs and Wistars were used. (1) Under urethane (0.8 g/kg, ip) anesthesia, a catheter was inserted into the bladder to instill reagents (2.4 ml/h) and to measure intravesical pressure. After detecting 4-5 micturition reflexes induced by saline instillation, GYY4137 solution (10-8, 10-7 and 10-6 M) or vehicle was instilled. (2) Bladder dome and trigone (BL-D and BL-T) were prepared from both rats. Effects of NaHS (1×10-8 to 3×10-4 M) were evaluated on carbachol (10-5 M)-induced pre-contracted bladder strips. H2S contents were measured by the methylene blue method. Expression levels of enzymes related to H2S biosynthesis [3-mercaptopyruvate sulfurtransferase (MPST), cysteine aminotransferase (CAT) and cystathionine β-synthase (CBS)] were examined by western blotting. RESULTS: (1) GYY4137 significantly prolonged intercontraction intervals compared to the vehicle-treated group in Wistars, but not in SHRs (Table 1). (2) NaHS-induced relaxation on pre-contracted BL-D and BL-T strips was significantly attenuated in SHRs compared to Wistars (Table 2). The H2S content in the bladder of SHRs was significantly higher than that of Wistars (Fig. C). The expression levels of MPST, but not CAT or CBS, in the SHR bladder were significantly higher than those in the Wistar bladder (Fig. D). CONCLUSIONS: H2S-induced bladder relaxation in SHRs is attenuated, which might be a cause of hypertension-mediated development of bladder dysfunctions. To compensate the less responsiveness, endogenous H2S levels might be increased in the SHR bladder. Therefore, the endogenous H2S system might be a new drug candidate for the treatment of hypertension-mediated bladder dysfunctions. Source of Funding: JSPS KAKENHI (#15K15583), GSK Japan Research Grant 2017 © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e423-e423 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Suo Zou* More articles by this author Takahiro Shimizu More articles by this author Shogo Shimizu More articles by this author Youichirou Higashi More articles by this author Takaaki Aratake More articles by this author Masaki Yamamoto More articles by this author Tomoya Hamada More articles by this author Yoshiki Nagao More articles by this author Masashi Honda More articles by this author Motoaki Saito More articles by this author Expand All Advertisement PDF downloadLoading ...