MP06-15 MYOFIBROBLASTS CYTOKINES SECRETED BY THE PROGRESSION OF BENIGN PROSTATIC HYPERPLASIA PROMOTE MONOCYTE RECRUITMENT AND INDUCE POLARIZATION OF THP1 TO M2-TYPE MACROPHAGES

Abstract

You have accessJournal of UrologyBenign Prostatic Hyperplasia: Basic Research & Pathophysiology (MP06)1 Apr 2020MP06-15 MYOFIBROBLASTS CYTOKINES SECRETED BY THE PROGRESSION OF BENIGN PROSTATIC HYPERPLASIA PROMOTE MONOCYTE RECRUITMENT AND INDUCE POLARIZATION OF THP1 TO M2-TYPE MACROPHAGES Muqiu Zhang* Muqiu Zhang*Muqiu Zhang* More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000820.015AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Introduction and Objective: Our previous studies, stroma–epithelium ratios and collagen deposition were prevalent in rapidly-progressed BPH patients (age ≤50 years old) . Meanwhile, we also detected that increased M2 macrophage infiltration in rapidly-progressed BPH patients, M2 macrophage induces myofibroblast phenotype during the progression of benign prostatic hyperplasia. The present study mainly investigates if myofibroblasts cytokines secreted by the progression of benign prostatic hyperplasia may promote monocyte recruitment and induce polarization of THP1 to M2-type macrophages. METHODS: Methods: We separated the human primary prostate fibroblast (PrPF) cells from the early progressed BPH (PrPF-early), age-matched prostate (PrPF-control), and elderly BPH tissues (PrPF-old). In vitro, we uesd flow cytometry to detect CD163 and CD206 (M2-type macrophages’ markers ) expression of THP-1 cells after co-culture 4days with three groups of human primary prostate fibroblast, respectively. Quantitative PCR was used to identify cytokines secreted by three groups of human primary prostate fibroblast. RESULTS: Results: THP-1 was recruited by human primary prostate fibroblast of rapidly-progressed BPH than in the other two groups. Significant increase in the percentage of M2 macrophages after co-culture with PrPF-early. Cytokines MCP-1 and IL-6 were increased in primary prostate fibroblast of rapidly-progressed BPH. Primary prostate fibroblast of rapidly-progressed BPH could recruit THP-1 and induce polarization of THP1 to M2-type macrophages. CONCLUSIONS: Conclusion: The early-progressed BPH fibroblasts recruit THP-1 and induce polarization of THP1 to M2-type macrophages. Source of Funding: no © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e56-e57 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Muqiu Zhang* More articles by this author Expand All Advertisement PDF downloadLoading ...