MP05-12 DEPENDENT ANTIBIOTIC AND OXALATE INTERACTIONS IMPACT ATHEROSCLEROSIS AND CALCIUM OXALATE STONES IN THE KIDNEYS

Abstract

You have accessJournal of UrologyCME1 May 2022MP05-12 DEPENDENT ANTIBIOTIC AND OXALATE INTERACTIONS IMPACT ATHEROSCLEROSIS AND CALCIUM OXALATE STONES IN THE KIDNEYS Jose Agudelo, Lucas Osborn, Beckey DeLucia, Mark Brown, Ava Adler, Jan Claesen, Jane Nguyen, and Aaron Miller Jose AgudeloJose Agudelo More articles by this author , Lucas OsbornLucas Osborn More articles by this author , Beckey DeLuciaBeckey DeLucia More articles by this author , Mark BrownMark Brown More articles by this author , Ava AdlerAva Adler More articles by this author , Jan ClaesenJan Claesen More articles by this author , Jane NguyenJane Nguyen More articles by this author , and Aaron MillerAaron Miller More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002522.12AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Previous studies have shown a positive relationship between cardiometabolic syndrome and increased risk of urinary stone disease (USD). However, the underlying mechanisms that leads to these co-morbidities are unknown. In the current study, we aimed to determine microbiome and diet dependent effects on an atherosclerosis and calcium oxalate stone formation in a mouse model. METHODS: Sixty-six female ApoE null mice were randomly assigned to either a basal diet or 3% oxalate diet. Half of the animals received antibiotic water and the other half sterile water. Mice consumed diets ad libitum for 12-weeks. At the end of the trial, fecal pellets were collected for 16S rRNA quantification. Mice were necropsied, and kidneys, aortas and liver were collected. Kidneys and aortas underwent polarized light quantification of oxalate content. Kidneys were also assessed for oxalate nephropathy after Von Kossa and Alizarin red staining and aortas were assessed for atherosclerosis plaque quantification. Livers were used for bile acid content assessment. Results were compared on a one-way and two-way ANOVA analyses. RESULTS: Bile acid production was significantly impacted by both oxalate and antibiotics. Renal histopathological analysis revealed that oxalate led to an increase in dilated tubules, oxalate nephropathy and tubular injury metrics, which was exacerbated with antibiotic use. Total atherosclerotic plaque lesions in the aortas and the presence of calcium oxalate in the plaque was significantly higher in the group that received dietary oxalate, which again was exacerbated by antibiotic use. CONCLUSIONS: Our results support the hypothesis that exogenous sources of oxalate may lead to both kidney stones and atherosclerosis through the formation of calcium oxalate plaques, which is exacerbated by a loss of function dysbiosis driven by long-term antibiotic use. Importantly, given the clear and significant formation of kidney stones, the APOE mice may serve as a more appropriate mouse model for kidney stones that what has previously been used. Source of Funding: Seed Funding Lerner Research Institute. Urology Care Foundation Research Scholar Award © 2022 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 207Issue Supplement 5May 2022Page: e72 Advertisement Copyright & Permissions© 2022 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jose Agudelo More articles by this author Lucas Osborn More articles by this author Beckey DeLucia More articles by this author Mark Brown More articles by this author Ava Adler More articles by this author Jan Claesen More articles by this author Jane Nguyen More articles by this author Aaron Miller More articles by this author Expand All Advertisement PDF DownloadLoading ...