Moxifloxacin superior to cefuroxime in reducing early‐phase adherence of staphylococcus epidermidis to hydrophobic intraocular lenses

Abstract

AbstractPurpose To evaluate the bacterial anti‐adhesive effect of cefuroxime and moxifloxacin on the primary‐attachment phase of S.epidermidis to acrylic intraocular lenses IOLsMethods 40 single‐piece SA30AT IOLs were used throughout the study. IOLs were divided into 5 groups. 2 groups were soaked in antibiotic solution,(moxifloxacin 0.5mg/0.1ml or cefuroxime 1mg/0.1ml),15 min before incubation in a S.epidermidis suspension 108 CFU/ml,t1.2 groups were incubated in the bacterial suspension for 15 min before being added with antibiotics,t2.The last group consisted in a control group. After 1 hour incubation at 37°C, for all groups, IOLs were removed from the test vial and rinsed before being sonicated and vortexed to remove the adhering bacteria. The resultant suspension was diluted and spread over a nutritive agar plate. Colonies were counted after 24‐hour incubation. Comparison among groups was performed using the Wald parametric statistical test. A p‐value <0.05 was considered statistically significant.Results Mean numbers of CFU/IOL was Cef‐t1:184.103[SE: 5,24 ; SD: 28,21],Cef‐t2:117.103[SE: 5,74 ; SD: 30,37], Mox‐t1:1,27.103[SE: 0,12 ; SD: 0,61], Mox‐t2:25.103[SE:1,98 ; SD: 9,72] and Ctrl:361.103[SE: 26,9 ; SD: 107,6].There was no significant difference in antibiotic time introduction concerning cefuroxime (p=0.132). Moxifloxacin was more effective when used before incubation (p<0.001). Overall, moxifloxacin was more effective than cefuroxime (p<0,001).Conclusion Both moxifloxacin and cefuroxime significantly reduced S.epidermidis adherence on IOLs.The anti‐adhesive effect was superior with moxifloxacine. Further studies are needed to confirm results on IOLs made of other biomaterials.