Abstract
Preventing the rapid spread of viral infectious diseases has become a major concern for global health. In this study, we present a microfluidic platform that performs an immunoassay of viral antigens in a simple, automated, yet highly sensitive manner. The device uses silica particles embedded with highly bright quantum dots (QD2) and performs the immunoassay with a vertically movable top layer and a rotating bottom layer. Through the motion of the layers and the surface tension in the liquids, reagents move from top chambers to bottom chambers and mix homogeneously. A tip in the top layer with a mobile permanent magnet moves the immune complexes comprising the magnetic beads, virus particles, and QD2 between the bottom chambers. In this way, our automated device achieves a highly sensitive magnetic bead-based sandwich immunoassay for the influenza A H1N1 virus within 32.5 min. The detection limit of our method is 5.1 × 10−4 hemagglutination units, which is 2 × 103 times more sensitive than that of the conventional hemagglutination method and is comparable to PCR. Our device is useful for the rapid and sensitive detection of infectious diseases in point-of-care applications and resource-limited environments.
Highlights
Virus detection on microfluidic platforms can be useful to prevent and control large outbreaks of infectious disease [1]
The sensitivity of the immunoassays for the presence of antibodies in human samples may depend on the viral titer and time of sample collected after viral infection; Compared with the viral hemagglutination assay with its limit of detection (LOD) of 1 hemagglutinating unit (HAU) [32], our method provides 2 × 103 times higher sensitivity
magnetic bead (MB) was successfully applied on a device with movable layers
Summary
Virus detection on microfluidic platforms can be useful to prevent and control large outbreaks of infectious disease [1]. QD-embedded silica particle (QD2), which has 500 times stronger photoluminescenc than individual QDs [18] This QD2 was successfully applied to a magnetic bead (MB)-based sandwich immunoassay for influenza A H1N1 (FluA/H1N1) virus detection with higher sensitivity yet in a manual manner [19]. This QD2 was successfully applied to a magnetic bead (MB)-based sandwich make the assay process automated, numerous microfluidic components and periphera immunoassay for influenza A H1N1 (FluA/H1N1) virus detection with higher sensitivity setups necessary. Realize sample-to-answer for virus anti a microfluidic device with open chambers in movable top and bottom layers. It did not realize sample-toIn thisdetection study, we present a microfluidic that isolates viralonly antigen and detect answer for virus antigens. On the device for virus detection using quantum dot probes
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
